Effect of chitosan-oligosaccharides on hydrophobicity of pathogenic Escherichia coli

Chitosan-oligosaccharides가 병원성 대장균의 소수성(疎水性)에 미치는 영향

  • Choi, Hyun-sung (College of Veterinary Medicine, Chonnam National University) ;
  • Han, Ho-jae (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Hee-kyung (Biological Engineering Institute, Chonnam National University) ;
  • Kim, Hee-sun (Biological Engineering Institute, Chonnam National University) ;
  • Kang, Mun-il (College of Veterinary Medicine, Chonnam National University)
  • 최현성 (전남대학교 수의과대학) ;
  • 한호재 (전남대학교 수의과대학) ;
  • 김희경 (전남대학교 생물공학연구소) ;
  • 김희선 (전남대학교 생물공학연구소) ;
  • 강문일 (전남대학교 수의과대학)
  • Received : 1999.04.12
  • Published : 1999.06.22

Abstract

The purpose of this study was to evaluate effect of chitosan-oligosaccharides (CHIOL) on hydrophobicity of pathogenic E coli including a field isolate from suckling piglet with diarrhea, E coli-O157 : H7, and E coli-O149 : K88ac. E coli field isolate appeared adhesion of 100% to n-hexadecane between 0.00125% and 0.05% CHIOL. E coli-O157 : H7 occurred adhesion of 69% and 64% under the level of 0.00125% and 0.025% CHIOL, respectively. E coli-O149 : K88ac showed adhesion of 100% in higher than 0.025% CHIOL. For cationic action, the adhesion of E coli isolate and E coli-O149 : K88ac to n-hexadecane were inhibited at level of higher than 10mM $Ca^{2+}$ but did not induce any difference among the concentrations used(p < 0.01). However, the adhesion of E coli-O157 : H7 to n-hexadecane was inhibited at level of higher than 50mM $Ca^{2+}$. In a field trial, control piglets showed average mortality of up to 58% during 3 days after the onset of diarrhea. In contrast, the prevalence of E coli-induced diarrhea in CHIOL-treated groups without mortality was dropped down to average 34% on the 1st day after the treatment of CHIOL, and average 2% on the 4th day. After then, piglets with diarrhea was not present. In conclusion, the low concentrations of CHIOL were most likely to associate with the enhancement of hydrophobicity to pathogenic E coli. Calcium inhibited the hydrophobicity of E coli by CHIOL. These results suggested that CHIOL could be played an efficient and reliable role in treating enteric colibacillosis of piglets.

Acknowledgement

Grant : 가축의 생산성 저해질병 예방 및 치료용 항미생물질의 개발

Supported by : 농진청