Diagnosis of canine distemper by in situ hybridization

In situ hybridization에 의한 개 디스템퍼의 진단

  • Cho, Hyeon (College of Veterinary Medicine, Chonnam National University) ;
  • Park, Nam-yong (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Yong-hwan (College of Veterinary Medicine, Chonnam National University) ;
  • Cho, Kyoung-oh (College of Veterinary Medicine, Chonnam National University) ;
  • Park, Hyung-seon (College of Veterinary Medicine, Chonnam National University) ;
  • Park, Young-seok (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Bong-joo (College of Veterinary Medicine, Chonnam National University) ;
  • Chung, Chi-young (College of Veterinary Medicine, Chonnam National University) ;
  • Im, Hyung-ho (College of Veterinary Medicine, Chonnam National University)
  • 조현 (전남대학교 수의과대학) ;
  • 박남용 (전남대학교 수의과대학) ;
  • 김용환 (전남대학교 수의과대학) ;
  • 조경오 (전남대학교 수의과대학) ;
  • 박형선 (전남대학교 수의과대학) ;
  • 박영석 (전남대학교 수의과대학) ;
  • 이봉주 (전남대학교 수의과대학) ;
  • 정치영 (전남대학교 수의과대학) ;
  • 임형호 (전남대학교 수의과대학)
  • Received : 1999.04.24
  • Published : 1999.06.22

Abstract

We have developed the in situ hybridization(ISH) technique for rapid diagnosis of canine distemper(CD) which is the major infectious disease in dogs. In our experiment, we rapidly detected distribution of the specific canine distemper viral genome without disrupting morphology of tissues or cells. Two oligonucleotide probes for ISH were synthesized chemically and labelled 5' end with nonisotopic biotin by DNA synthesizer. The whole procedures of ISH was completed within 1~2 hours using the Microcapillary action system. On histological study, typical cytoplasmic or intranuclear inclusion bodies were observed in the trachea, bronchiole, brain, and urinary bladder with the presence of prominent red positive signals on ISH, indicating specific CDV genome from the paraffin-embedded tissues of infected 13 cases. The results showed ISH can be used as a rapid and effective diagnostic method for diagnosis of CD.