Epidemiological analysis of Escherichia coli O157 : H7 by pulsed-field gel electrophoresis and multiplex polymerase chain reaction

  • Jung, Byeong-yeal (Department of Bacteriology, National Veterinary Research and Quarantine Service, MAF) ;
  • Jung, Suk-chan (Department of Bacteriology, National Veterinary Research and Quarantine Service, MAF) ;
  • Cho, Dong-hee (Department of Bacteriology, National Veterinary Research and Quarantine Service, MAF) ;
  • Kim, Jong-yeom (Department of Bacteriology, National Veterinary Research and Quarantine Service, MAF) ;
  • Kim, Bong-hwan (College of Veterinary Medicine, Kyungpook National University)
  • Received : 1999.04.20
  • Published : 1999.04.01

Abstract

Twenty three strains of Escherichia (E) coli O157 : H7 isolated from Korea, Japan, USA were analyzed by pulsed-field gel electrophoresis (PFGE) of XbaI-digested chromosomal DNA and multiplex polymerase chain reaction. Various PFGE patterns of E. coli O157 : H7 were found on the same farm. Most of the E, coli O157 : H7 strains had shiga-like toxin (slt) II gene only (43.5%) or both slt I and slt II genes(30.4%). eaeA gene was highly conserved in the E. coli O157 : H7. There was no correlation between PFGE and slt gene patterns. The results indicate that various genotypes of E. coli O157 : H7 have spread throughout the country and genomic DNA patterns generated by PFGE are highly specific for different strains and have significant value in epidemiologic investigations of infectious disease outbreaks.