Regulation of circulating Mg2+ in the rat by metabolic inhibition

흰쥐에서 대사작용 억제에 의한 혈중 Mg2+ 조절

  • Kim, Jong-shick (College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Shang-jin (College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Jin-shang (College of Veterinary Medicine, Chonbuk National University)
  • Received : 1999.01.07
  • Published : 1999.03.22

Abstract

Magnesium ($Mg^{2+}$) plays an important role in the regulation of a range of intracellular processes. Regulation of extracellular $Mg^{2+}$ contents was studied in the anesthetized Sprague-Dawley (SD) rats. Animals were injected intraperitoneally with sodium nitrite ($NaNO_2$), and circulating $Mg^{2+}$($[Mg^{2+}]c$) was measured after the injection and then 10 and 20 minutes later. A dose-dependent increase in $[Mg^{2+}]c$ was observed in animals injected with $NaNO_2$ at a dose of 10mg/kg or higher. Pretreatment with methylene blue prevented the $NaNO_2$-induced increase in $[Mg^{2+}]c$. $[Mg^{2+}]c$ displayed an inverse linear correlation with hemoglobin and exponential correlation during $NaNO_2$ injection. Injection of KCN or rotenone also induced an increase in $[Mg^{2+}]c$. An increase in $[Mg^{2+}]c$ was observed when respiration rate was reduced from 100/min (140ml/min) to 10/min (14ml/min) during 30 min. These results indicate that changes in $[Mg^{2+}]c$ inversely reflect alteration of ATP in a model of metabolic inhibition.

Acknowledgement

Supported by : 전북대학교 부속 생체안전성연구소 (CNU-BSRI)