Adhesion of Oral Pathogens to Human Submandibular-Sublingual Salivary Proteins

악하선-설하선 혼합타액내 주요 단백질에 대한 구강내 주요 균주의 부착

  • Lee, Sung-Woo (Dept. of Oral Medicine & Oral Diagnosis, College of Dentistry, Seoul National University) ;
  • Chung, Sung-Chang (Dept. of Oral Medicine & Oral Diagnosis, College of Dentistry, Seoul National University) ;
  • Kim, Young-Ku (Dept. of Oral Medicine & Oral Diagnosis, College of Dentistry, Seoul National University) ;
  • Kho, Hong-Seop (Dept. of Oral Medicine & Oral Diagnosis, College of Dentistry, Seoul National University)
  • 이승우 (서울대학교 치과대학 구강내과.진단학 교실) ;
  • 정성창 (서울대학교 치과대학 구강내과.진단학 교실) ;
  • 김영구 (서울대학교 치과대학 구강내과.진단학 교실) ;
  • 고홍섭 (서울대학교 치과대학 구강내과.진단학 교실)
  • Published : 1999.09.30

Abstract

The present study was performed to investigate the binding between salivary proteins(low-molecular-weight mucin;MG2, amylase, proline-rich proteins;PRPs) and oral pathogens(Streptococcus gordonii, Actinomyces viscosus, Staphylococcus aureus) by using solid-phase assay. In the case of transferring proteins to Immobilon-P, S. gordonii binds to MG2. A. viscosus binds to MG2, amylase, and PRPs, and S. aureus binds to MG2 and amylase. On nitrocellulose membrane, S, gordonii and A. viscosus bind to MG2, amylase, and PRPs. S. aureus binds to MG2 and PRPs. However, rabbit anti-A. viscosus antisera and rabbit anti-S. aureus antisera showed cross reactivity to PRPs adsorbed to only nitrocellulose membrane in negative control experiments, which were done without bacterial overlay. The results were different according to the membrane used as solid-phase, which reflected the assay-sensitive nature of binding experiment. PRPs and amylase are known to be components of tooth enamel pellicle. In addition, there was experimental evidence that PRPs and MG2 may covalently bind to oral mucosal epithelium. Considering above facts, the results of the present study can provide information on the interactions between salivary proteins and oral bacteria on tooth and oral mucosal surfaces.

Acknowledgement

Supported by : 서울대학교