Effects of Taeumin Chungsimyeunjatang on the Cerebral neurons injured by Hydrogen Peroxide

태음인(太陰人) 청심연자탕(淸心蓮子湯)이 Hydrogen Peroxide에 손상(損傷)된 백서(白鼠)의 대뇌신경세포(大腦神經細胞)에 미치는 영향(影響)

  • Ok, Yun-young (Department of Sasang Constitutional Medicine, Wonkwang University) ;
  • Ryu, Do-gon (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Kyung-yo (Department of Sasang Constitutional Medicine, Wonkwang University)
  • 옥윤영 (원광대학교 한의과대학 사상의학교실) ;
  • 유도곤 (원광대학교 한의과대학 생리학교실) ;
  • 김경요 (원광대학교 한의과대학 사상의학교실)
  • Published : 1999.12.31

Abstract

1. Purpose : The purpose of this study was to determine the effects of Chungsimyeunjatang on the cerebral neurons injured by hydrogen peroxide($H_2O_2$). 2. Methods : I observed cell viability in mouse cerebral neurons exposed to hydrogen peroxide by NR assay and MTT assay and determined lipid peroxidation and amounts of LDH release in mouse cerebral neurons exposed to hydrogen peroxide. After administration of Chungsimyeunjatang water extracts, I observed significant changes of cell viability, lipid peroxidation and amounts of LDH release in mouse cerebral neurons exposed to hydrogen peroxide. 3. Results : Hydrogen peroxide showed neurotoxicity. Cell viability in mouse cerebral neurons exposed to hydrogen peroxide decreased in NR assay and MTT assay. Lipid peroxidation and amounts of LDH release in mouse cerebral neurons exposed to hydrogen peroxide increased. Chungsimyeunjatang was very effective in blocking hydrogen peroxide-induced neurotoxicity.

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