Anticariogenic Effects of Unripe Apple Extract

애사과 추출물의 충치억제효과

  • 윤석영 ((주)한국야쿠르트 중앙연구소) ;
  • 김성훈 ((주)한국야쿠르트 중앙연구소) ;
  • 정해림 ((주)한국야쿠르트 중앙연구소) ;
  • 이정준 ((주)한국야쿠르트 중앙연구소) ;
  • 허철성 ((주)한국야쿠르트 중앙연구소) ;
  • 백영진 ((주)한국야쿠르트 중앙연구소)
  • Published : 2000.02.28


Anticariogenic activity of the unripe apple extract was studied by observing the inhibitory effects on GTase(glucosyltransferase) activity, cell adherence and acid production of Streptococcus mutans. Among the four S. mutans strains, S. mutans MT 8148 had the highest water-insoluble glucan forming activity. (+)-Catechin and tannic acid, the major components of the unripe apple polyphenols inhibited GTase activity by 60% at 1 mg/ml and 90% at 5 mg/ml. Tannic acid and unripe apple extract inhibited adherence ability of S. mutans by 50% and 30%, respectively. But the acid production of S. mutans was not influenced by the polyphenols. Disc diffusion test showed that the polyphenols have no antimicrobial activity against S. mutans, which indicates that the inhibition of GTase activity and cell adherence were not resulted from the cell growth inhibition. Our results convinced the possible application of the unripe apple extract as the anticariogenic food additives.