Effects of Ultrafiltration on the Production of Xylooligosaccharides

Xylooligosaccharides 제조를 위한 한외여과 처리

  • Park, Youn-Je (Department of Fermentation, Research and Development Center, TS Corporation) ;
  • Oh, Hwa-Gyun (Department of Fermentation, Research and Development Center, TS Corporation) ;
  • Lee, Ji-Wan (Department of Fermentation, Research and Development Center, TS Corporation) ;
  • Lee, Chang-Seung (Department of Fermentation, Research and Development Center, TS Corporation) ;
  • Lee, Un-Taek (Department of Fermentation, Research and Development Center, TS Corporation) ;
  • Rhew, Bo-Kyoung (Department of Fermentation, Research and Development Center, TS Corporation) ;
  • Yang, Chang-Kun (Department of Fermentation, Research and Development Center, TS Corporation) ;
  • Yoon, Se-Wang (Department of Fermentation, Research and Development Center, TS Corporation)
  • 박윤제 (대한제당(주) 중앙연구소) ;
  • 오화균 (대한제당(주) 중앙연구소) ;
  • 이지완 (대한제당(주) 중앙연구소) ;
  • 이창승 (대한제당(주) 중앙연구소) ;
  • 이운택 (대한제당(주) 중앙연구소) ;
  • 류보경 (대한제당(주) 중앙연구소) ;
  • 양창근 (대한제당(주) 중앙연구소) ;
  • 윤세왕 (대한제당(주) 중앙연구소)
  • Published : 2000.04.30

Abstract

Crude xylooligosaccharides(XO) were separated with a cellulose membrane (10,000 molecular weight cut-off). Flux variation was measured at transmembrane pressures of 5, 10, 20 and 30 psi, while flow rates were maintained at 100, 200, 300 and 400 ml/min and temperatures at 10, 20, 23-25 and $30^{\circ}C$. Optimal conditions were found at the transmembrane pressure of 20 psi, the flow rate of 300 ml/min and the temperature of $23-25^{\circ}C$, where the flux was decreasing with time by 62% after 200 min. Total sugar, total solid and ash contents were rather independent of ultrafiltration but protein was removed up to 55% from crude XO. Especially, the use of ultrafiltration was very effective for decolorization (84%) of crude XO. These results suggest that ultrafiltration is a useful method for the primary purification of XO.

Keywords

xylooligosaccharides;ultrafiltration;purification;decolorization