Culture Conditions on the Antimutagenic Effects of Lactobacillus plantarum KLAB21 isolated from Kimchi against N-methyl-N'-nitro-N-nitrosoguanidine and 4-nitroquinoline-1-oxide

김치에서 분리한 Lactobacillus plantarum KLAB21의 배양조건에 따른 N-methyl-N'-nitro-N-nitrosoguanidine과 4-nitroquinoline-1-oxide에 대한 항돌연변이 효과

  • Published : 2000.04.30

Abstract

Lactobacillus plantarum KLAB21 isolated from Kimchi has been reported to produce antimutagenic subtance(s) in the culture medium. In this study, antimutagenic effects of the strain KLAB21 were investigated to under various culture conditions maximize the production of antimutagenic substance(s) against N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) on Salmonella typhimurium TA100 and 4-nitroquinoline-1-oxide(NQO) on S. typhimurium TA98. Glucose(2%) as a carbon source and yeast extract(1%) as a nitrogen source resulted in the highest production of the antimutagenic substance(s) against both mutagens in the culture supernatant of L. plantarum KLAB21. The most effective concentrations of bactopeptone as a nitrogen source were 1% against MNNG and 1.5% against NQO. Optimal pH of the medium, culture temperature, and shaking speed for the antimutagenic substance(s) production were pH 7.0, $37^{\circ}C$ and 150 rpm, respectively. Under the optimal condition, the antimutagenic effects of L. plantarum KLAB21 culture supernatant were 98.4% against MNNG on S. typhimurium TA100 and 57.3% against NQO on S. typhimurium TA98.

Keywords

Lactobacillus plantarum KLAB21;antimutagenic effects;Kimchi;N-methyl-N'-nitro-N-nitrosoguanidine(MNNG);4-nitroquinoline-1-oxide(NQO)