Properties of Acid Tolerance of the Adipic acid-resistant Mutant of Leuconostoc paramesenteroides

Adipic acid 저항성 변이주 Leuconostoc paramesenteroides의 내산성 특성

  • Lee, Joong-Keun (Korea Health Industry Development Institute) ;
  • Lee, Hong-Seok (Korea Health Industry Development Institute) ;
  • Kim, Young-Chan (Korea Health Industry Development Institute) ;
  • Joo, Hyun-Kyu (Department of Biological Science, SunMoon University) ;
  • Lee, Si-Kyung (Department of Apply Biology and Chemistry, Kun-Kuk University) ;
  • Kang, Sang-Mo (Department of Microbiological Engineering, Kun-Kuk University)
  • 이중근 (한국보건산업진흥원) ;
  • 이홍석 (한국보건산업진흥원) ;
  • 김영찬 (한국보건산업진흥원) ;
  • 주현규 (선문대학교 응용생물과학부) ;
  • 이시경 (건국대학교 응용생물과학과) ;
  • 강상모 (건국대학교 미생물공학과)
  • Published : 2000.04.30

Abstract

Properties of acid tolerance of an adipic acid-resistant mutant, Leuconostoc paramesenteroides (ANaP100) were studied and compared with those of its paired wild type of Leu. paramesenteroides (LPw). The value of protons permeability of LPw after an acid shock at pH 5.0 was 4.3 min, while the value of ANaP100 was 4.8 min at the same pH. The maximal specific activities of ATPase of LPw and ANaP100 were 0.59 unit/mg protein and 0.63 unit/mg protein at pH 6.0, respectively. The release of magnesium ion from the mutant strain was about 27.3% at pH 4 after 2 hrs, while the wild strain was about 52.2% under the same conditions. The contents of $C_{19:0,cyclo}$ and $C_{18:1}$ in a membrane fatty acid of ANaP100 and LPw were higher and lower, respectively, than that of LPw. These results indicated that acid tolerance of ANaP100 was improved in comparison with that of its wild type, LPw.

Keywords

adipic acid;acid tolerance;lactobacilli;kimchi