Screening of Deoxynivalenol Producing Fungi from Greenhouse Horticulture by Enzyme Amplification System Immunoassay

Enzyme Amplification System Immunoassay에 의한 시설원예산물의 Deoxynivalenol 생성곰팡이의 검색

  • Park, Mi-Ja (Department of Food Science and Technology, Gyeongsang National University) ;
  • Park, Jung-Hyun (Department of Food Science and Technology, Gyeongsang National University) ;
  • Chung, Duck-Hwa (Department of Food Science and Technology, Gyeongsang National University)
  • Published : 2000.04.30

Abstract

In order to evaluate the safety of greenhouse horticulture products in Korea, we carried out this work by screening of Fusarium species, which produce deoxynivalenol (DON) from greenhouse horticulture in Western Gyeongnam and Northern Gyeongbuk, Korea. For this study, high sensitive enzyme-linked immunosorbent assay, ALP/NADP method, was applied to detection of DON by enzyme amplification system. From 192 samples of greenhouse horticulture soil and its products, 103 isolates of Fusarium species were obtained. The isolates were cultured at 28C for 15 days and the cultured mediums were extracted by ethyl acetate. The production of DON was verified by thin layer chromatography (TLC). As the results of TLC, 8 strains were identified as DON producing strain. We screened potential producers of DON by ALP/NADP. The levels of DON production were shown from 0.007 to 1.21 g/ml of YES medium. The maximum DON producing strain No. 32-D-3 was isolated from soil in Namhae, Korea. In conclusion, the above results indicate that DON producing fungi contaminated greenhouse horticulture products in Korea. Therefore, further studies are required to accumulate more detailed data about the contamination of DON in various cereals.

Keywords

deoxynivalenol(DON);enzyme amplification system immunoassay