Investigation of in vitro Antigenotoxic Effect of Korean Mistletoe(Viscum album coloratum) Using Comet Assay

Comet assay에 의한 한국산 겨우살이(Viscum album coloratum)의 항유전독성 규명

  • Park, Jong-Heum (Institute of Functional Foods and Safety, Handong University) ;
  • Ji, Seung-Taek (Institute of Functional Foods and Safety, Handong University) ;
  • Hyun, Chang-Kee (School of Bioscience and Food Technology, Handong University) ;
  • Chin, Koo-Bok (Biotechnology Research Institute, Chonnam National University) ;
  • Shin, Heuyn-Kil (School of Bioscience and Food Technology, Handong University)
  • 박종흠 (한동대학교 식품 기능성 및 안전성 연구소) ;
  • 지승택 (한동대학교 식품 기능성 및 안전성 연구소) ;
  • 현창기 (한동대학교 생물식품공학부) ;
  • 진구복 (전남대학교 생명공학연구소) ;
  • 신현길 (한동대학교 생물식품공학부)
  • Published : 2000.04.30

Abstract

To investigate the antigenotoxicity of Korean mistletoe using Comet assay, the crude extract was divided into 4 fractions, i.e. fraction I (MW range over 14,000), fraction II $(8,000{\sim}14,000)$, fraction III $(3,500{\sim}8,000)$, and fraction IV (below 3,500) by molecular weight fractionation. In the non-tumoral 3T3 cells, fraction IV could reduce DNA damage induced by MNNG in a dose dependent pattern while fraction I and III which were known to contain lectins and viscotoxins, respectively, did not show the activity. By heat treatment, the antigenotoxic activity of faction IV, though was gradually diminished according to heating time, was found to be maintained significantly. From the Sephadex G-25 gel filtration chromatography, a more purified fraction responsible for the activity of faction IV was obtained from the latter part of total elute. Therefore, it was concluded that the antigenotoxic components of Korean mistletoe were water soluble substances of MW below 1,000 and there is a possibility of utilization as a material of functional foods for chemoprevention.

Keywords

Korean mistletoe;antigenotoxicity;Comet assay;DNA damage;chemoprevention