Antimicrobial activity of Specific IgY against Streptococcus mutans

Streptococcus mutans에 대한 specific IgY의 항균력

  • Kim, Young-Boong (Division of Livestock product utilization, Korea Food Research Institute) ;
  • Rho, Jeong-Hae (Division of Livestock product utilization, Korea Food Research Institute) ;
  • Shon, Dong-Hwa (Division of Livestock product utilization, Korea Food Research Institute) ;
  • Kim, Hee-Joo (Division of Livestock product utilization, Korea Food Research Institute) ;
  • Seong, Ki-Seung (Division of Livestock product utilization, Korea Food Research Institute) ;
  • Lee, Nam-Hyung (Division of Livestock product utilization, Korea Food Research Institute)
  • Published : 2000.12.30

Abstract

Antimicrobial effects of the specific IgY separated from eggs which were laid by hens vaccinated with Streptococcus mutans were investigated. The comparison tests of vaccination, addition levels of crude specific IgY, and innoculation concentration were applied by microscopic observation and turbidity test. Ten% addition of crude specific IgY obtained from vaccinated hens showed agglomerative clusters of S. mutans cells in supernatants and sediments, while crude IgY produced by non-vaccinated hens showed no cluster. IgY addition above 5% showed agglutinating clusters of most S. mutans cells and there was definite difference between IgY addition below 2.5% and above 5%. Concentration tests of crude IgY revealed that antimicrobial effects were differentiated by addition level and addition over 10% produced satisfactory results with turbidity test. The cluster size was dependent upon concentration of crude IgY addition. $10^5\;cfu/mL$ inoculation showed agglutinated cells and extent of agglomeration was proportional to cell numbers. Study of inoculation levels showed that 10% addition of crude IgY decreased turbidity effectively regardless of number of S. mutans cells. Plaque formation decreased to 75% with 15% addition of specific IgY concentration. These results implied that IgY separated from eggs laid by S. mutans-vaccinated hens might prevent dental caries caused by S. mutans.

Keywords

streptococcus mutans;specific IgY;antimicrobial activity