Isolation and Identification of Active Antimicrobial Substance against Listeria monocytogenes from Ruta graveolens Linne

운향으로부터 Listeria monocytogenes에 대한 항균 활성 물질의 분리 및 구조동정

  • Ahn, Yong-Seon (Faculty of Biotechnology(Food Science & Technology Major), Chonbuk National University) ;
  • Shin, Dong-Hwa (Faculty of Biotechnology(Food Science & Technology Major), Chonbuk National University) ;
  • Baek, Nam-In (Department of Agriculture, Kyung Hee University)
  • 안용선 (전북대학교 응용생물공학부(식품공학, 농업과학기술연구소)) ;
  • 신동화 (전북대학교 응용생물공학부(식품공학, 농업과학기술연구소)) ;
  • 백남인 (경희대학교 농학과)
  • Published : 2000.12.30

Abstract

Ethanol extracts from Ruta graveolens Linne exhibited strong antimicrobial activities by disc diffusion method against 5 strains of Listeria monocytogenes (ATCC 19111, ATCC 19112, ATCC 19113, ATCC 19114 and ATCC 15313). Ethanol extract from Ruta graveolens Linne was subsequently fractionated by n-hexane, chloroform, ethyl acetate and water. Chloroform fraction of Ruta graveolens Linne showed strong growth inhibition at concentrations as low as 40 ppm level in broth culture medium against 5 strains of L. monocytogenes for 72 hr at $30^{\circ}C$. Single substance(RTG1-1) was isolated by silica gel column chromatography from chloroform fraction of Ruta graveolens Linne. RTG1-1 showed a strong bactericidal activity against L. monocytogenes at a concentration of 20 ppm level. Purified RTG1-1 was identified as gravacridonechlorine by analyses of EI-Mass, $^1H-NMR$ and $^{13}C-NMR$.

Keywords

Ruta graveolens Linne;antimicrobial activity;gravacridonechlorine;Listeria monocytogenes