The Effects of Rhus Extracts on The Cytotoxicity on Cancer Cells and E6 and E7 Oncogenes of Human Papillomavirus Type 16

옻 추출물의 세포독성 및 자궁 경부암 바이러스 암 유발인자 E6 와 E7의 작용에 미치는 효과

  • Cho, Young-Sik (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Joung, Ok (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Cho, Cheong-Weon (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Lee, Kyung-Ae (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Shim, Jung-Hyun (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Kim, Kwang-Soo (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Lee, Hong-Soo (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology) ;
  • Seung, Ki-Seung (Korea Food Research Institute) ;
  • Yoon, Do-Young (Cellular Biology Lab, Korea Res. Institute of Bioscience and Biotechnology)
  • Published : 2000.12.30

Abstract

Cervical cancer has been one of the leading causes of female death from cancer worldwide with about 500,000 deaths per year. A strong association between certain human papillomaviruses (HPV types 16 and 18) and cervical cancer has been well known. An extract of natural products, Rhus, has been used to investigate whether this agent has the ability of inhibiting the oncogenes E6 and E7 of HPV type 16. This Rhus inhibited the proliferation of human cervical cancer cell lines (C-33A, SiHa, Caski) and HaCaT keratinocytes in a dose response manner. In vitro binding assay and ELISA showed that Rhus inhibited the in vitro binding of E6 and E6AP which are essential for the binding and degradation of the tumor suppressor p53. In addition, Rhus inhibited the in vitro binding of E7 and Rb which essential tumor suppressor for the control of cell cycle. The level of mRNA for E6 was also decreased by Rhus while that of E7 mRNA was not changed. Our data suggested that Rhus inhibited the oncogenecity of E6 and E7 of HPV 16 type, thus can be used as a putative anti-HPV agent for the treatment of cervical carcinomas by HPV.

Keywords

Rhus;HPV;E6;E7;E6AP