Anticariogenic effects of Coptis chinensis Franch Extract

황련(Coptis chinensis Franch) 추출물의 항충치효과

  • Jang, Gui-Hyun (Department of Food Science and Technology, Chonbuk National University) ;
  • Ahn, Byung-Young (Professional Graduated School of Oriental Medicine, Wonkwang University) ;
  • Oh, Suk-Heung (Division of Bioscience and Biotechnology, Woosuk University) ;
  • Choi, Dong-Seong (Division of Bioscience and Biotechnology, Woosuk University) ;
  • Kwon, Yong-Ju (Department of Food Science and Technology, Chonbuk National University)
  • 장귀현 (전북대학교 식품공학과) ;
  • 안병용 (원광대학교 한의학 전문대학원) ;
  • 오석흥 (우석대학교 생명공학부) ;
  • 최동성 (우석대학교 생명공학부) ;
  • 권용주 (전북대학교 식품공학과)
  • Published : 2000.12.30

Abstract

The effects of Coptis chinensis Franch(CCF) extract on the growth, acid production, cell adherence and glucosyltransferase(GTase) activity of Streptococcus mutans JC-2 were investigated. Methanol extract from CCF showed a strong inhibitory effect on the growth of S. mutans. The minimal inhibition concentration(MIC) of the methanol extract was determined as $130\;{\mu}g/mL$, whereas MIC of water extract was $200\;{\mu}g/mL$. MICs of berberine and palmatine were $50\;{\mu}g/mL$ and $110\;{\mu}g/mL$, respectively, showing stronger antimicrobial activities than the extracts of CCF. Antimicrobial activities of methanol extract, berberine and palmatine were not decreased by heating at $121^{\circ}C$ for 15 min, suggesting that the antimicrobial components including berberine and palmatine are heat-stable. Acid production of S. mutans was decreased by methanol and water extracts, berberine and palmatine. The activity of GTase was inhibited by methanol extract, berberine and palmatine at $300\;{\mu}g/mL$ with 23.2%, 46.1% and 17.1%, respectively, but was not inhibited by water extract. The water extract and palmatine at sub-MICs inhibited the adherence of S. mutans to glass surface by 59.2% and 41.7%, respectively. These results suggest that CCF extracts have anticariogenic effects and could be used as an anticariogenic food additive.

Keywords

Coptis chinensis Franch;berberine;palmatine;anticariogenic;Streptococcus mutans