Formation of Acycloretinoic Acid by Autoxidation of Lycopene

Lycopene의 자동산화에 의한 Acycloretinoic acid의 생성

  • Kim, Seon-Jae (Food Industrial Technology Research Center, Mokpo National University)
  • 김선재 (목포대학교 식품산업기술연구센터)
  • Published : 2000.12.30

Abstract

Acycloretinoic acid was prepared from acycloretinal by oxidation with Tollens reagent. Acycloretinoic acid was separated with Silica-HPLC and analyzed by ODS-HPLC with a photodiode array detector and by GC-MS. Lycopene was solubilized in toluene and aqueous Tween 40, and then oxidized by incubating at $37^{\circ}C$ under atmospheric oxygen. Acidic compound was produced by autoxidation of lycopene. Retention time, UV-Vis spectra and mass spectra of the acidic compound were identical to the standard acycloretinoic acid. Thus, acycloretinoic acid was confirmed to occur in vitro under oxidation condition of lycopene.