Hepatic Detoxification activity and reduction of Serum Alcohol concentration of Hovenia dulcis $T_{HUNB}$ from Korea and China

중국산과 국내산 헛개 나무 열매의 체내 알콜 분해능 및 간 해독 작용

  • Kim, Min-Hae (Division of Food and Biotechnology, Kangwon National University) ;
  • Chung, Yoo-Taek (Division of Food and Biotechnology, Kangwon National University) ;
  • Lee, Jin-Ha (Division of Food and Biotechnology, Kangwon National University) ;
  • Park, Young-Shik (Biotechnology Research Institute of MiRae Bio, Chunchon Bio-Industry Venture Support Center) ;
  • Shin, Myung-Ki (Biotechnology Research Institute of MiRae Bio, Chunchon Bio-Industry Venture Support Center) ;
  • Kim, Ho-Sang ;
  • Kim, Dong-Hoon ;
  • Lee, Hyeon-Yong (Division of Food and Biotechnology, Kangwon National University)
  • 김민회 (강원대학교 식품생명공학부) ;
  • 정우택 (강원대학교 식품생명공학부) ;
  • 이진하 (강원대학교 식품생명공학부) ;
  • 박영식 ((주) 미래바이오 생명공학연구소, 춘천생물산업벤쳐지원센터) ;
  • 신명기 ((주) 미래바이오 생명공학연구소, 춘천생물산업벤쳐지원센터) ;
  • 김호상 ;
  • 김동훈 ;
  • 이현용 (강원대학교 식품생명공학부)
  • Published : 2000.09.30

Abstract

There was not noticeable differences in decreasing blood alcohol concentrations between Korea and China-produced Hovenia dulcis $T_{HUNB}$, showing only 1-2 % higher decreasing rate for Korea-produced seed extracts than those from China. It was also found that the blood alcohol decreasing ability was greatly enhanced by partitioning the crude extracts produced from both places. The both extracts (crude and partitioned) accelerated the reducing rate of blood alcohol concentrations down to 1-2 hours, compared to that of control (taking only ethanol). The crude extracts from imported seeds seemed to have slightly better effect on improving in vivo ADH and ALDH activities than domestic ones; however, not for partitioned extracts. It was interesting that the partitioned extracts from both countries enhanced ADH enzyme activity up to 60% than the crude, compared to the control, while ALDH activity was not much affected by the partitioned extracts. It was also confirmed that both ADH and ALDH activities were well balanced in controlling blood alcohol concentration maintaining 28-29% of enzyme activities in vivo. The extracts proved to have better effect on enhancing ALDH activity than ADH activity, which is one of possible explanation that Hovenia dulcis $T_{HUNB}$ can effectively relieve the hangover by fast decreasing acetaldehyde concentration in the liver and blood. GST activity was also increased in the range of 120 to 300% by adding crude or partitioned extracts from both countries; however, there was no difference in enhancing GST activity between the extracts from two countries. The extracts showed competitive inhibition with GST activity, showing the reduction of enzyme activity at higher than 0. 6 (g/L) of the imported extracts.

Keywords

Serum alcohol concentration;Hepatic detoxification;Hovenia dulcis $T_{HUNB}$