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Molecular Biological Study of Anti-cancer Effects of Bee Venom Aqua-acupuncture

봉독약침(蜂毒藥鍼)의 항암효과(抗癌效果)에 대한 분자생물학적(分子生物學的) 연구(硏究)

  • Park, Chan-Yol (Dept. of Acupuncture & Moxibustion Oriental Medical College, KyungHee University) ;
  • Seo, Jung-Chul (Dept. of Acupuncture & Moxibustion Oriental Medical College, KyungHee University) ;
  • Choi, Do-Young (Dept. of Acupuncture & Moxibustion Oriental Medical College, KyungHee University) ;
  • Ahn, Byoung-Choul (Dept. of Acupuncture & Moxibustion Oriental Medical College, KyungHee University)
  • Published : 2000.07.30

Abstract

To study anti-cancer effect and molecular biological mechanism of bee venom for aqua-acupuncture, the effects of bee venom on cell viability and apoptosis were analyzed using MTT assay, tryphan blue assay, $[^3H]$thymidine release assay, flow cytometric analysis, and activity of caspase-3 protease activity assay. To explore whether anti-cancer effects of bee venom are associated with the transcriptional control of gene expression, quantitative RT-PCR analysis of apoptosis-related genes was performed. The obtained results are summarized as follows: 1. The MTT assay demonstrated that cell viability was decreased by bee venom in a dose-dependant manner. 2. Significant induction of apoptosis was identified using tryphan blue assay, $[^3H]$thymidine release assay, and flow cytomet1 ric analysis of sub $G_1$ fraction. 3. In analysis of caspase-3 protease activity, the activity had increased significantly, in a dose-dependant manner. 4. Quantitative RT-PCR analysis of the apoptosis-related genes showed that Bcl-2 and Bcl-$X_L$ were down-regulated whereas Bax was up-regulated by bee venom treatment.

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