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A Study on Major Components of Bee Venom Using Electrophoresis

전기영동법(Electrophoresis)을 이용한 봉약침의 주요 성분에 관한 연구

  • Lee, Jin-Seon (Department of Acupuncture and Moxibustion SangJi Oriental Medicine Hospital, SangJi University) ;
  • Kwon, Gi-Rok (Department of Acupuncture and Moxibustion SangJi Oriental Medicine Hospital, SangJi University) ;
  • Lee, Seung-Bae (Division of applied animal sciences, SangJi University)
  • Published : 2000.12.30

Abstract

This study was designed to study on major components of various Bee Venom(Bee Venom by electrical stimulation in Korea; K-BV I, Bee Venom by Microwave stimulation in Korea; K -BV II, 0.5rng/ml, Fu Yu Pharmaceutical Factory, China; C-BV, 1mg /ml, Monmouth Pain Institute, Inc., U.S.A.; A-BV) using Electrophoresis. The results were summarized as follows: 1. In 1:4000 Bee Venom solution rate, the band was not displayed distinctly usmg Electrophoresis. But in 1: 1000, the band showed clearly. 2. The results of Electrophoresis at solution rate 1:1000, K-BV I and K-BVII showed similar band. 3. The molecular weight of Phospholipase $A_2$ was known as 19,000 but its band was seen at 17,000 in Electrophoresis. 4. Protein concentration of Bee Venom by Lowry method was different at solution rate 1:4000 ; C-BV was $250{wmu}g/ml,\;K-BV\;I\;was\;190{wmu}g/ml,\;K-BVII\;was\;160{wmu}/ml\;and\;C-BV\;was\;45{wmu}/ml5$. Electrophoresis method was unuseful for analysis of Bee Venom when solution rate is above 1:4000 but Protein concentration of Bee Venom by Lowry method was possible. These data from the study can be applied to establish the standard measurement of Bee Venom and prevent pure bee venom from mixing of another components. I think it is desirable to study more about safety of Bee Venom as time goes by.

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  1. Stability and Safety of Bee Venom with and without Additives vol.32, pp.3, 2015, https://doi.org/10.13045/acupunct.2015045