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Effects of Hominis Placenta Aqua-acupuncture on Kidney and Liver Intoxicated by HgCI2 in Rats

자하거약침자극(紫河車藥鍼刺戟)이 HgCI2로 손상(損傷)된 흰쥐의 신장(腎臟) 및 간장(肝臟)에 미치는 영향(影響)

  • Lee, Sang-Keel (Dept. of Acupuncture & Moxibustion. College of Oriental Medicine, Kyung Hee University) ;
  • Seo, Jung-Chul (Dept. of Acupuncture & Moxibustion. College of Oriental Medicine, Kyung Hee University) ;
  • Choi, Do-Yong (Dept. of Acupuncture & Moxibustion. College of Oriental Medicine, Kyung Hee University) ;
  • Park, Dong-Suk (Dept. of Acupuncture & Moxibustion. College of Oriental Medicine, Kyung Hee University) ;
  • Lee, Yun-Hoo (Dept. of Acupuncture & Moxibustion. College of Oriental Medicine, Kyung Hee University) ;
  • Kang, Sung-Keel (Dept. of Acupuncture & Moxibustion. College of Oriental Medicine, Kyung Hee University)
  • Published : 2000.12.30

Abstract

Objective: This study was perfomled to examine the therapeutic effect of aqua-acupuncture solution of Hominis Placenta(HP) on kidney and liver intoxicated by $HgCI_2$ in rats. Methods: $10\%$ and $25\%$ HP aqua-acupuncture were carried out everyday for 8 days on corresponding bilateral loci of Shinsu(BL23) and Kansu(BL18), respectively, after mercuric chloride intoxication in rats. Thereafter BUN, creatinine, GOT, GPT, ALP, ${\gamma}$-GT, albumin and total bilirubin were measured before intoxication, and at the 4th and the 8th experimental day. Histopathological and immunochemical observation were also carried out. Results: 1. It showed significant decreases of BUN in the group of $10\%$ HP aqua-acupuncture into Shinsu on the 4th experimental day as compared with the control group. 2. It showed significant decreases of creatinine in the group of $10\%$ HP aqua-acupuncture into Shinsu on the 4th and the 8th experimental days as compared with the control group. 3. There were not any significant changes of GOT, GPT, ALP,${\gamma}$-GT, albumin and total bilirubin in the HP aqua-acupuncture groups compared with the control group. 4. By the histopathological observations on kidney under a light microscope, alt the $10\%$ and $25\%$ HP aqua-acupuncture into Shinsu showed the preventive effect on tubulo-interstitial necrosis and muItifocal calcification in tubular lumen respectively compared with the control group. 5. By the histopathological observations on liver under a light mIcroscope, the groups $10\%$ and $25\%$ HP aqua--acupuncture into Kansu did not show any significant changes in the liver compared with the control group. 6. By the immunochemical analysis of heat shock protein(hsp) and glucose-regulated protein(grp) in rat renal cortex, the expressions of hsp70 and grp78 were decreased in the $10\%$ and $25\%$ HP aqua-acupuncture into Shinsu respectively compared with the control group. Conclusion: These results suggest that Hominis Placenta aqua-acupuncture have an effect on prevention and protection of renal intoxication by $HgCI_2$ in rats.

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