The effect of fibrinolytic enzyme produced from Bacillus subtilis K-54 on the thrombosis and stress in vivo.

Bacillus subtillis K-54가 생산하는 Fibrinolytic enzyme의 혈전생성 및 스트레스에 미치는 영향

  • Published : 2000.02.01


The effect of fivrinolytic enzyme produced from Bacillus subtilis K-54 on the thrombosis and stress in vivo was investigated. Each partially purified fibrinolytic enzyme of 4 protein casein unit was administered orally for 3 days before intravenously injection with collagen and epinephrine. In the mice group administered with the enzyme and increased life span of mice was observed in comparison with that of control. The result suggest that the enzyme may prevent the formation of thrombos in vivo. Administration of the enzyme did not influence to stress itself because 5-hydroxyindoleacetatic acid concentration of brain in the mice group with stress did not decreased after the administration of the enzyme. The value of lipid peroxide (LPO) of the liver and brain cells in the group treted with the enzyme was lower than that of control. However, protein degradation (PDP value showed no significant difference between treatment and control groups. In addition, the value of activated partial thromboplastin time (APTT), protrombin time (PT0 and antiplasmin in blood were higher in the stress group than that of the enzyme treated group.



  1. Fact on File v.16 Arteriosclerosis, thrombosis & vascular biology Aarno, H.;T. P. Toronard
  2. Trends Biotechnol. v.7 Industrial enzymology: a look towards the future Arbige, M.;W. H. Pitcher
  3. Adv. Gerontol. Res. v.2 Lipid peroxidation-its measurement, occurrence and significance in animal tissue Barber, A. A.;F. Bernheim
  4. Blood v.62 Additional glycoprotien defects in Bernard Soulier's syndrom.: confirmation of genetic basis by parental analysis Berndt, M. C.;C. Grerogy;B. M. Chang;H. Zola;P. A. Castaldi
  5. FASEB J. v.2 no.10 Modulation of the hydrophobicity of glutamine synthetase by mixed-function oxidation Cervera, J;R. L. Levine
  6. Brit. J. Pharmacol. v.39 Rapid method for the determination of 5-hydroxytryptamine and hydroxyindole acetic acid in small reagens of brain Curzon, G.;A. R. Green
  7. J. Appl. Physiol. v.4 Fibrinolytic response to acute physical activity in older hypertensive men Desouza, C. A.;D. R. Dengel;M. A. Rogers;R. F. Macko
  8. J. Pharma. Exp. Therap. v.225 no.1 Mouse antithrombotic assay: A simple method for the evalution of antithrombotic agents in vivo. Potentiation of antithrombotic activity by ethyl alcohol Diminno, G.;M. J. Silver
  9. Thromb. Haemostasis v.72 no.5 Evaluation of recently described tests for detection of the lupus anticoagulant Forastiero, R. R.;G. S. Cerrato;L. O. Carreras
  10. Biol. Pharm. Bull. v.18 no.9 Transport of nattokinase across the rat intestinal tract Fujita, M.;H. Kyungsu;Y. Ito;S. Misawa;S. Nishimuro
  11. J. Nat'l. Cancer Inst. v.86 Reduced risk of esophageal cancer associated with green tea consumption Gao, Y. T.;J. K. McLaughlin;W. J. Blot;J. F. Graumeni
  12. Acta. Obstet. Gynecol. Scand. v.54 Studies on coagulation and fibrinolysis in pregnancy Ghonnaess, H.;M. K. Fagerhol
  13. Angiologia v.43 The relationship between lipid peroxidation and platelets aggregation in atherosclerotic pationts Gomez, C. C.;R. Simoncarballo;A. C. Coma;T. Sanchez de Leon;D. E. Montero;P. R. Rodriguez
  14. Med. Clin. North. Am. v.65 Haemostasis, thrombosis and thromboembolic disorders Harlan, J. M.;L. A. Harker
  15. Thromb. Res. v.20 Effect of proteases on plasminogen activator release from isolated perfused dog leg Hijikata, A.;M. Hirata;H. Kitaguchi
  16. Diabetes Res. Clin. Pract. v.14 Increased inositol phosphate accumulation in platelets from patients with NIDDM Ishii, H.;F. Umeda;T. Hashimoto;H. Nawada
  17. Arter. Throm. Vascul. Biol. v.16 Association of clonic stress with plasminogen activator inhibitor-1 in healthy middle age men Katri, R.;R. Lassila;L. Keltikangas;Hautanen
  18. J. Free Radic. Biol. Med. v.2 Intracellular Proteolytic Systems may Function as Secondary Antioxidant Defense: An Hyphothesis Kelvin, J.;A. Davies
  19. Act. Med. Scand. v.219 Evaluation of platelet reactivity in diabetic mellitus Kutti, J.;H. Wadenvik;B. Hanestam;G. Stenstorm
  20. J. Biol. Chem. v.258 no.19 Oxidative modification of glutamine synthetase.Ⅰ. Inactivation is due to loss of one histidine residue Levine, R. L.
  21. Throm. Res. v.76 Studies of the platelet fibrinogen receptor in Glanzman patients and uremic patients Lindahl, T. L.;J. Lundahl;C. Netre;N. Egberg
  22. Int'l Arch. Occup. Environ. Health v.68 Relationship between job stress and plasma fibrinolytic activity in male Japanese workers Masao, I.;I. Tsuritani;Y. Noborisaka;Y. Yamada;H. Nakagawa
  23. The 3rd Int'l symp. on green tea Effect of anaerobic treated tea on the blood pressure of spontaneously hypertensive rats Masashi, O.
  24. Am. J. Cardiol. v.69 Platelet leucocyte-endothelial interaction in coronary artery disease Mehta, J. L.;F. A. Nicolini;W. H. Donnellu;W. W. Nichols
  25. J. Biochem. v.103 Elevation of cytosolic free calcium is directly evoked by Thromboxane A2 in human platelets during activation with collagen Moriyama, T.;H. Yakamura;H. Narita;K. Tanaka;T. Matsuur;M. Kito
  26. J. Pharmacol. v.48 Foot shock induced stress decrease leu-enkephalin immunoreactivity in rahypothalamus Roisser, J. R.;G. F. Bloom
  27. Semin. Hematol. v.22 Platelets and Willebrands disease Ruggeri, E. M.;T. S. Zimmerman
  28. Scand. J. Lab. Inv. v.37 Determination of a ner rapid plasmin inhibitor in human blood by means of a plasmin specific tripeptide substrate Teger-Nilsson, A. C.;P. Friberger;E. Gyzander
  29. Nature v.263 Selective activation of the mesocortical DS system by stress Thirry, A. M.;J. P. Tassin;G. Blanc;J. Glowinski
  30. Biochem. Biophys. Acta. v.797 Interaction of human platelet membrane glycoprotein with collagen and lectins Tsunehisa, S. T.;H. Tsuju;H. Tohyama;T. Osawa
  31. Eur. J. Clin. Invest. v.22 Hypersensitivity to ADP platelets from diabetic rats associated with enhanced fibrinogen binding Winocur, P. D.;D. W. Pery;R. L. Klnlough-Ruthbone
  32. Biochem. Med. v.15 A simple fluorometric assay for lipoperoxide in blood plasma Yagi, K.
  33. Kor. J. Appl. Microbiol. Biotechnol. v.26 Purification and characterization of Fibrinolytic enzyme excreted by Bacillus subtilis K-54 isolated from Chung Guk Jang Yoo, C. K.;W. S. Seo;C. S. See;S. M. Kang