Production and Characterization of Cyclodextrin Glucanotransferase fronm Bacillus sp. JK-43 Isolated from Kimchi

김치 분리균인 Bacillus sp. JK-43이 생산하는 Cyclodextrin Glucanotransferase의 생산 및 특성

  • Jun, Hong-Ki (Division of Biological Science, Pusan National University) ;
  • Bae, Kyung-Mi (Division of Biological Science, Pusan National University) ;
  • Kim, Young-Hee (Division of Biological Science, Pusan National University) ;
  • Baik, Hyung-Suk (Division of Biological Science, Pusan National University)
  • 전홍기 (부산대학교 생명과학부) ;
  • 배경미 (부산대학교 생명과학부) ;
  • 김영희 (부산대학교 생명과학부) ;
  • 백형석 (부산대학교 생명과학부)
  • Published : 2000.02.01


A bacterial strain, designated as JK-43, producing extracellular cyclodextrin glucanotransferase (CGTase)[EC] was isolated from kimchi. The CGTase from isolated strain JK-43 showed the transglucosylation activity from soluble starch to L-ascorbic acid(AA) compared to those obtained from other strains. A main product formed by this reaction was identified as $2-O-{\alpha}-glucopyranosyl$ L-ascorbic acid(AA-2G) by testing its susceptibility to ${\alpha}-glucosidase$ hydrolysis, the HPLC profiles, and through the elementary analysis. the ${\beta}-CD,\;{\gamma}-CD$, potato starch and corn starch were identified to be suitable glucosyl donor for transglucosylation reaction on AA by CGTase. Acceptor specificity on AA-2G production was examined by use of AA, Iso-AA and AA-2P. Transglucosylation was observed toward AA-2P as well as AA and Iso-AA. The microorganism isolated from kimchi was identified as a strain of Bacillus sp. JK-43 based on the morphological, cultural, biochemical characteristics and partial 16SrDNA sequence analysis. The maximal CGTase production was observed in a medium containing 1.0% soluble starch, 1.0% yeast extract, 1.0% $Na_2CO_3\;0.1%\;K_2HPO_4,\;and\;0.02%\;MgSO_4{\cdot}7H_2O$ with initial pH 7.0. The strain was cultured at $37^{\circ}C$ for 26 hrs with reciprocal shaking.


  1. Crit. Rev. Food Sci. Nutr. v.342 Biochemical, microbiological, and nutritional aspects of kimchi (Korean fermented vegetable products) Cheigh, H.S.;Park, K.Y.
  2. Korean J. Diet. Cult. v.4 Effect of some materials on the content of nirate, nitrite and vitamin C in kimchi during fermentation Lee, S.W.;Woo, S.J.
  3. Korean J. Appl. Microbiol. Biotechnol. v.20 Microfloral changes of the lactic acid bacteria during kimchi fermentation and identification of the isolates Lee, C.W.;Ko, C.Y.;Ha, D.M.
  4. Kor. J. Appl. Microbiol. Biotechnol. v.25 Evaluation of optimum conditions for the electrofusion between Lactobacillus sp.JC-7 isolated from kimchi and Lactobacillus acidophilus 88 Jo, Y.B.;Choi, H.J.;Baik, H.S.;Jun, H.K.
  5. J.Korean Soc. Food Nutr. v.24 The nitritional evaluation and antimutagenic and anticancer effects of kimchi Park, K.Y.
  6. J. Korean Soc. Food Sci. Nutr. v.26 Changes in chlorophylls and carotenoids of mustard leaf kimchi during fermentation and their antioxidative activities on the lipid oxidation Song, E.S.;Jeon, Y.S.;Cheigh, H.S.
  7. J. Food. Soc. Nutr. v.1 A study on buchu (Leek, Allium odorum) kimchi Changes in chemical, microbial, sensory properties, and antimutagenicity of buchu kimchi during fermentation Lee, K.I.;Jung, K.O.;Rhee, S.H.;Suh, M.J.;Park, K.Y.
  8. J. Food Sci. Nutr. v.3 Formation of a L-ascorbic acid 2-0-α-glucoside during Kimchi fermentation Jun, H.K.Bae, K.M.;Kim, Y.H.;Cheigh, H.S.
  9. J.Microbiol. Biotechnol. v.8 Identification of L-ascorbic acid 2-0-α-glucoside, a stable form of ascorbic acid, in kimchi Jun, H.K.;Bae,, K.M.;Kim, Y.H.
  10. Nucleic Acids Res. v.12 Ascorbate stimulation of PAT cells causes an increase in transcription rates and a decrease in degradation rates of procollagen mRNA Lyons, B.L.;Schwarz, R.I.
  11. Ann. N.Y. Acad. Sci. v.258 Chemistry and metabolism of ascorbic acid and ascorbate sulfate Tolbert,B.M.;Downing, M.;Carlson, R.W.;Knight, M.K.;Baker, E.M.
  12. Antiviral. Res. v.27 In vitro inhibition of human cytomegalovirus replication in human foreskin fibroblasts and endothelial cells by ascorbic acid 2-phosphate Cinatl, J.;Cinatil, J.;Weber, B.;Rbenau, H.;Gumbel, H.O.;Chenot, J.F.;Scholz, m.;Encke, A.;Doerr, H.W.
  13. Vitamin v.41 Chemistry and application of ascorbic acid phosphate Mima, H.;Nomura, H.Imai, Y.;Takashima, H.
  14. Biochemistry v.8 The occurrence of ascorbic acid sulfate in brine shrimp, Artemia salina Mead, C.G.;Finamore, F.J.
  15. J. Agric. Food Chem. v.32 Synthesis of the 2-methylether of L-ascorbic acid: stability, vitamin activity, and carbon-13 nuclear magnetic resonance spectrum compared to those of the 1- and 3-methyl ethers Lu, P.W.;Lillard, Jr.D.W.;Seib, P.A.;Kramer, K.J.;Liang, Y.T.
  16. Chem. Pharm. Bull. v.38 L-Ascorvic acid α-glucoside formed by resioselective transglucosylation with rat intestinal and rice seed α-glucosidase : its improved stability and structure determination Yamamoto, L.;Muto, N.;Murakami, K.;Suga, S.;Yamaguchi, H.
  17. Biochem. Pharmacol. v.44 Comparison of ascorbic acid ascorbic acid 2-O-α-glucoside on the cytotoxicity and bioavailability to low density cultures of fibroblasts Murakami, K.;Muto, N.;Fukazawa, K.;Yamamoto, I.
  18. J. Nutr. Sci. Vitaminol. v.38 Vitamin C activity of 2-O-α-D-glucopyranosyl-L-ascorbic acid in guinea pig. Wakamiya, H.;Suzuki, E.;Yamamoto, I.;Akiba, M.;Otsuka, M.;Arakawa, N.
  19. J.Nutr. v.122 Collagen synthesis in human skin fibroblasts is stimulated by a stable form of ascorbate, 2-O-α-D-glucopyranosyl-L-ascorbic acid Yamamoto, I.;Muto, N.;Murakami, K.;Akiyama, J.
  20. J. Pharmacobiodyn. v.13 Antiscorbutic activity of L-ascorbic acid 2-glucoside and its availability as a vitamin C supplement in normal rats and guinea pig Yamamoto, I.;Suga, S.;Mitoh, Y.;Tanaka, M.;Muto, N.
  21. J.Biochem. v.107 Enzymatic formation of a non-reducing L-ascorbic acid α-glucoside : purification and properties of α-glucosidase catalyzing site-specific transglucosylation from rat small intestine Muto, N.;Nakamura, T.;Yamamoto, I.
  22. Int. J.Vit. Nutr. Res. v.62 Evaluation of ascorbic acid 2-O-α-glucoside as vitamin C source : Mode of intestinal hydrolysis and absorption following oral administration Muto, N.;Terasawa, K.;Yamamoto, I.
  23. Biochem. Biophys. Acta. v.1035 Formation of a stable L-ascorbic acid α-glucoside by mammalian α-glucosidase-catalyzed transglucosylation Yamamoto, I.;Muto, N.;Nagata, E.;Nakamura, T.;Suzuki, Y.
  24. Agric. Biol. Chem. v.55 Synthesis of 2-O-α-D-glucopyranosyl L-ascorbic acid by cyclomaltodextrin glucanotransferase from Bacillus stearothermophilus Hajime, A.;Masaru, Y.;Shuzo, S.;Itaru, Y.
  25. J.Korean Soc. Food Sci. Nutr. v.27 Some properties and optimal culture conditions of cyclodextrin glucanotransferase of Bacillus sp. S-6 isolated from kimchi Jun, H.K.;Jo, Y.B.;Kim, S.J.;Bae, K.M.
  26. Vitamins v.43 Enzymatic formation of new L-ascorbic acid glucosidase (in Japanese) Miyake, T.;Suzuki, Y.
  27. Vitamins v.47 Biosynthesis of ascorbic acid glucoside (in Japanese) Suzuki, Y.;Miyake, T.;Uchida, K.;Mino, A.
  28. Vitamins v.63 Enzymatic formation of L-glucosylascorbic acid (in Japanese) Suzuki, Y.;Uchida, K.
  29. Enzyme Mocrob. Technol. v.22 Bacterial cyclodextrin glucanotransferase Tonkova, A.
  30. Appl. Biochem. Biotechnol. v.43 Synthesis of cyclodextrin glucosyltransferase by Bacillus cereus for the production of cyclodextrins Jamuna, R.;Saswatbi, N.;Sbeela, R.;Ramakrishna,S.V.
  31. Appl. Microbiol. Biotechnol. v.36 Production of cylodextrin glucanotransferase of Bacillus circulans var. alkalophilus ATCC21783 in B.subtilis Paloheimo,M.;Haglund, D.;Aho, S.;Korhola, M.
  32. J. Ferment. Bioeng. v.70 Purification and properties of cyclodextrin glycosyltransferase from Bacillus sp. AL-6 Fujita, Y.;Tsubouchi, H.;Lnagi, Y.;Tamita, K.;Ozaki, A.;Nakanishi, K.
  33. Agric. Biol. Chem. v.40 Characterization and some cultural conditions of a cyclodextrin glycosyltransferase-producing alkaloophilic Bacillus sp. Nakamura, N.;Horikoshi, K.
  34. Biotecnol. Bioeng. v.19 Production of Schardinger β-dextrin by soluble and immobilized cyclodextrin glycosyltransferase of an alkalophilic Bacillus sp. Nakamura, N.;Horikoshi, K.
  35. Biochemistry v.7 Purification and properties of the amylase of Bacillus macerans DePinto, J.A.;Campbvell, L.I.
  36. Agric. Biol. Chem. v.38 Purification and some properties of cyclodextrin glycosyltransferase from a strain of Bacillus species Kitakata, S.;Tsuyama, N.;Okada, S.
  37. Biokhimiya v.60 A new cyclomaltodextrin glucanotransferase from halophilic Bacillus (in Russian) Abelian, V.A.;Adamian, M.O.;Abelian, L.A.;Balayan, A.M.;Alrikian, E.K.
  38. Biochimica. Biophysica. Acta. v.1078 Characterization of Bacillus stearothermophilus cyclodextrin glucanotransferase in acorbic acid 2-O-α-glucoside formation Mari, T.;Norio, M.;Itaru, Y.
  39. Kor. J. Appl. Microbiol. Biotechnol. v.26 Purification and charterization of β-cylodextrin glucanotransferase excreted by Bacillus firmus var.alkalophilus Shin, H.D.;Chan, K.;Lee, Y.H.