Production of 2-O--$\alpha$-D-Glucopyranosyl L-Ascorbic Acid by Cyclodextrin Glucanotransferase from Bacillus sp. JK-43

Bacillus sp. JK-43의 Cyclodextrin Glucanotransferase에 의한 2-O-$\alpha$-D-Glucopyranosyl L-Ascorbic Acid 생산에 관한 연구

  • 전홍기 (부산대학교 생명과학부) ;
  • 배경미 (부산대학교 생명과학부) ;
  • 김영희 (부산대학교 생명과학부) ;
  • 김성구 (부경대학교 생물공학과)
  • Published : 2000.02.01

Abstract

The 2-O-$\alpha$-D-glucopyranosyl L-ascorbic acid (AA-2G) which was enzymatically glucosylated with the cyclodextrin glucanotransferase (CGTase) [EC 2.4.1.19] from Bacillus sp. JK-43 has been reported previously. The presnet experiments examined the optimal conditons for the productio of AA-2G from AA and soluble starch, and characterized the properties of the CGTase from Bacillus sp. JK-43. The reaction mixture for the maximal production of AA-2G was followings; 12% total substrate concentration, 1,400 usits/mL of CGTase and a mixing ratio of 2 : 3(g or AA : g of soluble starch). Under this condition, 1.76mM of AA-2G, which corresponded to 2.53% yield based on AA, was produced after incubation for 24hrs at 45$^{\circ}C$ (pH 5.5). The optimum pH and temperature for the CGTase activity were 6.0 and 45$^{\circ}C$, respectively. The enzyme was stable at pH 5.5 to 9.5, and at temperature up to 5$0^{\circ}C$. The thermostability of the enzyme could be enhanced up to 6$0^{\circ}C$ by the addition of 30mM CaCl2.

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