Analysis of some pesticides in urine by GC/MS

GC/MS를 이용한 뇨중 몇가지 농약의 분석

  • Park, Seong-Soo (Division of Natural Medicinal Products, Korea Food and Drug Administration) ;
  • Park, Song-Ja (Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology) ;
  • Pyo, Hee-Soo (Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology) ;
  • Cho, Jung-Hee (Division of Natural Medicinal Products, Korea Food and Drug Administration) ;
  • Kim, Hye-Soo (Division of Natural Medicinal Products, Korea Food and Drug Administration) ;
  • Park, Taek-Kyu (Dept. of Chemistry, Konkuk University)
  • 박성수 (식품의약품안전청 생약제제과) ;
  • 박송자 (한국과학기술연구원 생체대사연구센타) ;
  • 표희수 (한국과학기술연구원 생체대사연구센타) ;
  • 조정희 (식품의약품안전청 생약제제과) ;
  • 김혜수 (식품의약품안전청 생약제제과) ;
  • 박택규 (건국대학교 이과대학 화학과)
  • Published : 2000.03.31

Abstract

The purpose of this study is to establish the assessment techniques of hazardous chemicals by the development of analytical method of biological samples. In this study, we have developed an extraction method of nine pesticides used for rice paddy that resulted in high recovery from the spiked human urine by the liquid-liquid extraction with diethyl ether at pH 7.0. Calibration curve obtained from each pesticide standard using by gas chromatography/mass spectrometry/selected ion monitoring has shown good linearity and detection limits were the range of $0.4{\sim}2.0$ ng/mL in urine. As a biological monitoring, urine samples of local farmers exposed directly to nine pesticides in the field were collected and analyzed by GC/MS. Of the tested pesticides, metabolites of phenthoate assumed were identified by GC/MS analysis. No parent compound was detected.