Spectrophotometric microtitre assay for rapid screening of membrane-disrupting herbicides

분광분석을 이용한 막과산화작용 제초제의 신속한 검정법

  • Kwon, Ok-Kyung (Screening Research Division, Korea Research Institute of Chemical Technology) ;
  • Cho, Kwang-Yun (Screening Research Division, Korea Research Institute of Chemical Technology) ;
  • Kim, Jin-Seog (Screening Research Division, Korea Research Institute of Chemical Technology)
  • 권옥경 (한국화학연구소 농약활성연구팀) ;
  • 조광연 (한국화학연구소 농약활성연구팀) ;
  • 김진석 (한국화학연구소 농약활성연구팀)
  • Published : 2000.03.31

Abstract

This study was conducted to establish an efficient screening method of membrane-disrupting herbicides, based on the result that membrane leakage leading to evolution of carbonyl compounds and aldehydes could be colorimetrically assessed by formation of stable chromophores with MBTH and ferric chloride. Assay conditions including plant material, light intensity, irradiation time, concentrations of reagents for colorization, and reaction time were optimized, and activities of different types of known herbicides were investigated with cucumber cotyledon discs. That result indicated that this assay was sensitive to membrane-disrupting herbicides, such as protoporphyrinogen oxidase inhibitors (acifluorfen, oxyfluorfen, oxadiazon) and an electron interceptor from photosystem I(paraquat). With seven compounds newly synthesized, highly positive correlation was observed between herbicidal activities measured by greenhouse bioassay and MBTH method. Because only a single disc(4 mm diameter) from cucumber cotyledon in 60 ${\mu}L$ of incubation solution was required to assay, a large number of herbicides could be tested simultaneously using 96-well microtitre plates. But the assay efficiency is not likely to significantly increase because of the boiling step during the reaction.