Determination of Optimum pH of Enzyme by Electrochemical Method

전기화학적 방법에 의한 효소의 최적 pH 결정

  • Received : 1999.10.12
  • Published : 2000.02.25

Abstract

A biosensor for the determination of hydrogen peroxide was constructed by immobilizing of porcine small instestinal tissue in a plain carbon paste, and the effect of varying the $H_2O_2$ concentration and pH on the rate of catalytic reaction was evaluated. For the mathematical simplicity, no mediator was added. Electrochemical properties and the maximal rate could be derived from the quantitative analysis of the observed phenomena related to the electrode reaction. Also, pH dependence of the Michaelis constant enabled to calculate various thermodynamic parameters and subsequently to design a electrochemical method to determine the optimum pH of enzyme.

Keywords

biosensor;hydrogen peroxide;porcine peroxidase;optimum pH

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