Determination of Optimum pH of Enzyme by Electrochemical Method

전기화학적 방법에 의한 효소의 최적 pH 결정

  • 윤길중 (청주대학교 이공대학 화학과) ;
  • 이범규 (조선대학교 자연과학대학 화학과) ;
  • 권효식 (충북대학교 사범대학 과학교육과)
  • Received : 1999.10.12
  • Published : 2000.02.25


A biosensor for the determination of hydrogen peroxide was constructed by immobilizing of porcine small instestinal tissue in a plain carbon paste, and the effect of varying the $H_2O_2$ concentration and pH on the rate of catalytic reaction was evaluated. For the mathematical simplicity, no mediator was added. Electrochemical properties and the maximal rate could be derived from the quantitative analysis of the observed phenomena related to the electrode reaction. Also, pH dependence of the Michaelis constant enabled to calculate various thermodynamic parameters and subsequently to design a electrochemical method to determine the optimum pH of enzyme.


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