Spinach Root-Tissue Based Amperometric Biosensor for the Determination of Hydrogen Peroxide

시금치 뿌리 조직 바이오센서를 이용한 과산화수소의 정량

  • Lee, Beom-Gyu (Division of Physics and Chemistry, Chosun University) ;
  • Yoon, Kil-Joong (Department of Chemistry, Chongju University) ;
  • Kwon, Hyo-Shik (School of Science Education, Chungbuk National University)
  • 이범규 (조선대학교 자연과학대학 물리화학부) ;
  • 윤길중 (청주대학교 이공대학 화학과) ;
  • 권효식 (충북대학교 사범대학 과학교육연구소)
  • Received : 2000.03.16
  • Published : 2000.06.25

Abstract

The response characteristics of the bioelectrode developed by the co-immobilization of spinach root tissue and ferrocene in a carbon paste matrix for the amperometric determination of hydrogen peroxide were evaluated. In the range of electrode potential examined (-0.3~0.0V vs. Ag/AgCl). the response time was relatively short ($t_{95%}=11.8$ sec) and it responded in the wide range of pH. Also, its detection limit was $2.25{\times}10^{-6}M$ (S/N=3) and a relative standard deviation of the measurements which were repeated 15 times using $1.0{\times}10^{-3}M$ hydrogen peroxide was 1.87%. The bioelectrode sensitivity decreased to 40% of the original value in 19 days of continuous use.

Keywords

Bioelectrode;Spinach root tissue;Amperometric detennination of hydrogen peroxide

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