Induction of Boiling Stable Proteins by Cold and ABA Treatment in Radish Cotyledon and Hypocotyl

무 자엽과 하배축에서 저온과 ABA처리로 유도된 중탕에 강한 단백질 분석

  • 조봉희 (수원대학교 생명과학부)
  • Received : 2000.01.18
  • Published : 2000.06.25

Abstract

Boiling stable proteins of 53 kDa and 29 kDa existed natively in the cotyledons of Bak Kyoung, fall radish (Raphanus raphanistrodes L.) Boiling stable proteins of 36 kDa and 16.5 kDa were newly induced by cold stress and the proteins of 53 kDa and 29 kDa increased during the cold stress. The proteins of 53 kDa were denatured within 2 hrs after removing cotyledons from plants. Boiling stable proteins of 53 kDa existed natively in the hypocotyls as much as in the cotyledons whereas 24 kDa and 18 kDa proteins were increased by stress. Boiling stable proteins of 53 kDa were induced and those of the 25 kDa and 23 kDa were increased by cold treatment and ABA treatment in the cotyledons of Jangchundaehyung F1 spring white (Raphanus raphanistrodes L.). These results showed the differences of induced boiling stable proteins between fall radishes and spring radishes. Cycloheximide inhibited the induction of 25 kDa and 23 kDa proteins during stress. 22 kDa native protein disappeared during ABA treatment and reappeared by cycloheximide treatments. It may be explained that cycloheximide was responsible for the destruction process of proteins in the living organisms. The profile of boiling stable proteins in hypocotyls of spring radishes during stress was same as that of fall redishes.

Keywords

Radish;Abscisic acid;Cotyledon;Hypocotyl;Boiling stable protein

Acknowledgement

Supported by : 학술진흥재단

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