Simultaneous Determination of Biliary Free and Phospholipid Fatty Acids Using Gas Chromatography-Mass Spectrometry

GC-MS를 이용한 담즙내 유리 지방산 및 인지질 지방산들의 동시 분석

  • Yang, Yoon Jung (Bioanalysis & Biotransformation Research Center, Korea Institute of Science and Technology) ;
  • Lee, Seon Hwa (Bioanalysis & Biotransformation Research Center, Korea Institute of Science and Technology) ;
  • Chung, Bong Chul (Bioanalysis & Biotransformation Research Center, Korea Institute of Science and Technology)
  • 양윤정 (한국과학기술연구원 생체대사연구센터) ;
  • 이선화 (한국과학기술연구원 생체대사연구센터) ;
  • 정봉철 (한국과학기술연구원 생체대사연구센터)
  • Received : 2000.07.11
  • Published : 2000.10.25


The concentration of free fatty acids and fatty acid composition as well as cholesterol supersaturation in bile may be an important factor in the gallstone formation. Therefore, we simultaneously determinded 23 fatty acids in bile by selected ion monitoring (SIM) method of gas chromatography-mass spectrometry (GC-MS). Biliary fatty acids were extracted by aminopropyl column and the extracts with (phospholipid fraction) or without (free fatty acid fraction) alkaline hydrolysis of phospholipid were derivatized with MSTFA/TMCS (N-methyl-N-trimethylsilyl-trifluoroacetamide/trimethylsilylchloride) mixture in order to be detected on the GC-MS. The recovery range of this method was 61.1-99.0% and the RSD value of within-a-day and day-to-day test were 3.1-25.6% and 3.8-27.0%, respectively. Using this method, biliary profile was investigated in the bile of normal controls and patients with gallstones. The amounts and their distribution of free and phospholipid fatty acids showed different pattern between normal subjects and patients.


  1. J. Chromatogr. B. v.675 no.2 T.G. Bernhardt;P.A. Cannistraro;D.A. Bird;K.M. Doyle;M. Laposata
  2. 분석과학 v.11 no.374 양윤정;이선화;정봉철
  3. Scand. J. Gastroenterol. v.31 no.273 D. Jungst;R.D. Pozo;S. Christoph;J.F. Miquel;M.I. Eder;V. Lange;E. Frimberger;C.V. Ritter;G. Paumgrtner
  4. Dig. Dis. Sci. v.39 no.1313 A.J. Moser;M.Z. Abedin;J.J. Roslyn
  5. J. Chromatogr. B. v.653 no.1 S. Tazuma;S. Hatsushika;G. Yamashita;N. Aihara;M. Sasaki;K. Horikawa;Y. Yamashita;K. Teramen;H. Ochi;N. Hirono;H. Miura;T. Ohya;H. Hino;G. Kajiyama
  6. 대한화학회지 v.42 no.266 양윤정;이선화;김태욱;정봉철
  7. Hepatology v.16 no.71 F. Berr;E. Schreiber;U. Frick
  8. J. Lipid Res. v.33 no.1871 J.J. Agren;A. Julkunen;I. Penttila
  9. Hepatology v.16 no.960 F. Berr;J. Holl;D. Jungst;S. Fischer;W.O. Richter;B. Seifferth;G. Paumgartner
  10. J. Lipid Res. v.26 no.135 M.A. Kaluzny;L.A. Duncan;M.V. Merritt;D.E. Epps
  11. Surgery v.118 no.3 T.H. Magnuson;K.D. Lillemoe;R.C. High;H.A. Pitt
  12. Biochim. Biophys. Acta v.1167 no.142 S. Tao;S. Tazuma;G. Kajiyama
  13. Metabolism v.41 no.22 R.H. Dowling;S.H. Hussaini;G.M. Murphy;G.M> Besser
  14. J. Lipid Res. v.30 no.719 D.M. Heuman
  15. 분석과학 v.12 no.265 양윤정;이선화;정봉철
  16. 분석과학 v.11 no.429 이용문;문동철;한건
  17. Liver v.12 no.107 Z. Halpern;G. Goldman;Y. Peled;F. Konikoff;J. Rattan;D. Lichtenberg;T. Gilat
  18. J. Lipid Res. v.22 no.404 J. Ahlberg;T. Curstedt;K. Einarsson;J. Sjovall