Homogeneous Enzyme-linked Binding Assay for Mistletoe Lectin I Based on the Mistletoe Lectin I/D-galactose Interaction

Mistletoe lectin I/D-galactose의 인식결합에 기초한 Mistletoe lecti I에 대한 용액상 효소결합분석법에 관한 연구

  • 이인숙 (서울여자대학교 자연과학대학 화학과) ;
  • 이은아 (서울여자대학교 자연과학대학 화학과) ;
  • 전종순 (서울여자대학교 자연과학대학 화학과)
  • Received : 2000.09.27
  • Published : 2000.10.25

Abstract

A simple and rapid homogeneous enzyme-linked binding assay for mistletoe lectin I(ML I) was developed using a coupled enzyme system of malate dehydrogenase (MDH) and D-galactose. A highly substituted MDH-galactose conjugate was prepared by employing an isothiocyanate method for formation of thiourea bond. In the presence of ML I, ML I inhibits the activity of the conjugate based on the ML I/D-galactose specific interaction. Thus, the concentration of ML I can be related to the homogeneous inhibition of the MDH-galactose conjugate. Using this method. ML I can be measured at the level of microgram per milliliter within 10 minutes.

Keywords

mistletoe lectin I;D-galactose;homogeneous assay;enzyme-linked binding assay

Acknowledgement

Supported by : 서울여자대학교

References

  1. Principles and Practice of Immunoassay(2nd Ed.) v.389 P.J. Wood;G. Barnard;C.P. Price(ed.);D.J. Newman(ed.)
  2. Arzneim. -Forsch. Drug Res. v.43 no.2 J. Beuth;H.L. Ko;L. Tunggal;J. Geisel;G. Pulverer
  3. Anal. Chem. v.56 no.920A D. Monroe
  4. Arzneim. -Forsch. Drug Res. v.45 no.905 C. Jaggy;H. Musielski;K. Urech;G. Schaller
  5. Essential Immunology(4th ed.) I. Roit
  6. Biochem. J. v.195 no.481 H. Franz;P. Ziska;A. Kindt
  7. Dtsch. Zschr. Onkol. v.23 no.1 T. Hajto;K. Hostanska;M. Fornalski;A. Kirsch
  8. Biochim. Biophys. Acta v.567 no.66 E.F. Ullman;R.A. Yoshida;J.I. Blakemore;E. Maggio;R. Leute
  9. Anal. Chem. v.62 no.2663 B. Kim;G.S. Cha;M.E. Meyerhoff
  10. Anal. chem. v.57 no.12 R.H. William;H. Brian
  11. Immunoassay v.355 R.E. Geiger;D. Gabrijelcic;W. Miska;F.P. Diamandis(ed.);T.H. Christopoulos(ed.)
  12. Enzyme Immunoassay Heterogeneous enzyme immunoassays and their applications V. Alister
  13. Advances in Lectin Research v.4 no.33 H. Franz;H. Franz(ed.)