Effects of Some Crude Drug Extracts on the Brain Neurotransmitters in the Ethanol-Treated Rats

수종의 생약 추출물이 에탄올 투여 흰쥐의 뇌 부위별 신경전달물질에 미치는 영향

  • Received : 2000.09.01
  • Published : 2000.10.25


The concentration of neurotransmitters in rat brain was determined by HPLC-ECD (electrochemical detection) method and the effects of methanol extracts of some crude drugs, such as Polygala Radix, Myristicae Semen, Zizyphi Semen, Acori graminei Rhizoma, Visci Herba, Liriopsis Tuber, Myrrha on the concentration of neurotransmitters in the ethanol-treated rat brain were investigated. By the administration of ethanol, dopamine (DA), 3, 4-dihydroxyphenyl acetic acid (DOPAC) and serotonin (5-HT) levels in frontal cortex and 5-HT level in hippocampus were significantly increased compared with the neurotransmitter levels in the brain of saline-treated rats. The ${\gamma}$-aminobutyric acid (GABA) level in frontal cortex was decreased by the same treatment. There was a tendency that the DA level in frontal cortex and striatum of ethanol-treated rats were increased by the administration of crude drug extracts. Especially, Myrrha and Visci Herba significantly increased the DA level of frontal cortex in ethanol-treated rats, while they significantly decreased the 5-HT level in the same region of the brain. GABA level in striatum of ethanol-treated rats was significantly decreased by Myristicae Semen, Visci Herba and Myrrha. These results suggest that the tested crude drug extracts have selective interaction with neurotransmitters in specified region of central nervous system.


Supported by : 충남대학교


  1. J. Ethnopharmacol. v.42 no.2 C. Van Gils;P.A. Cox
  2. Chem. Pharm. Bull. v.45 no.7 M. Yoshikawa;T. Murakami;A. Ikebata;S. Wakao;N. Murakami;H. Matsuda;J. Yamahara
  3. J. Ethnopharmacol. v.61 no.3 J.F. Liao;S.Y. Huang;Y.M. Jan;L.L. Yu;C.F. Chen
  4. J. Neurochem. v.50 no.3 A.W. Procter;A.M. Palmer;P.T. Francis;S.L. Lowe;D. Neary;E. Murphy;R. Doshi;D.M. Bowen
  5. Neurosci. v.27 no.437 R. Balazs;O.S. Jorgensen;N. Hack
  6. J. Neurol. Sci. v.92 no.1 P. Herregodts;M. Bruyland;J. De Keyser;C. Solheid;Y. Michotte;G. Ebinger
  7. Brain v.122 M.A. Piggott;E.F. Marshall;N. Thomas;S. Lloyd;J.A. Court;E. Jaros;D. Burn;M. Johnson;R.H. Perry;I.G. McKeith;C. Ballard;E.K. Perry
  8. Mov. Disord. v.12 no.6 J.N. Joyce;G. Smutzer;C.J. Whitty;A. Myers;M.J. Bannon
  9. Maps and guide to microdisection of the rat brain M. Palkovits;M. Brownstein
  10. Trends Neurosci. v.10 no.288 G.L. Collingridge;T.V. Bliss
  11. J. Nat Prod v.62 no.7 T. Miyase;H. Noguchi;X.M. Chen
  12. Phytother Res. v.13 no.4 O.A. Olajide;F.F. Ajayi;A.I. Ekhelar;S.O. Awe;J.M. Makinde;A.R. Alada
  13. Science. v.24 no.1694 D. Muller;M. Joly;G. Lynch
  14. Chem. Pharm. Bulㅣ. v.47 no.12 H. Matsuda;T. Murakami;A. Ikebata;J. Yamahara;M. Yoshikawa
  15. Chem. Pharm. Bull. v.42 no.11 Y. Ikeya;K. Sugama;M. Maruno
  16. Brain v.111 S.L. Lowe;P.T. Francis;A.W. Procter;A.M. Palmer;A.N. Davison;D.M. Bowen