Determination of Polybrominated Biphenyls in Biota Samples Using Gas Chromatography/Mass Spectrometry

기체크로마토그래피/질량분석법을 이용한 생체시료 중 Polybrominated Biphenyls의 분석법

  • Hong, Jongki (Mass Spectrometry Team, Korea Basic Science Institute) ;
  • Baek, In-Girl (Mass Spectrometry Team, Korea Basic Science Institute) ;
  • Kim, Hyub (Mass Spectrometry Team, Korea Basic Science Institute) ;
  • Kim, Do-Gyun (Mass Spectrometry Team, Korea Basic Science Institute) ;
  • Seo, Jung-Joo (Mass Spectrometry Team, Korea Basic Science Institute) ;
  • Seo, Jong-Bok (Mass Spectrometry Team, Korea Basic Science Institute) ;
  • Park, Hyun-Mee (Advanced Analytical Center, Korea Institute of Science and Technology) ;
  • Lee, Kang-Bong (Advanced Analytical Center, Korea Institute of Science and Technology)
  • 홍종기 (기초과학지원연구소 질량분석팀, 정밀구조분석팀) ;
  • 백인걸 (기초과학지원연구소 질량분석팀, 정밀구조분석팀) ;
  • 김협 (기초과학지원연구소 질량분석팀, 정밀구조분석팀) ;
  • 김도균 (기초과학지원연구소 질량분석팀, 정밀구조분석팀) ;
  • 서정주 (기초과학지원연구소 질량분석팀, 정밀구조분석팀) ;
  • 서종복 (기초과학지원연구소 질량분석팀, 정밀구조분석팀) ;
  • 박현미 (한국과학기술연구원, 특성분석센터) ;
  • 이강봉 (한국과학기술연구원, 특성분석센터)
  • Received : 2000.07.20
  • Published : 2000.10.25

Abstract

The present study describes an analytical method for the determination of polybrominated biphenyls (PBBs) in biota samples by gas chromatography-mass spectrometry (GC/MS). PBBs are extracted twice from 20 g samples with mixture solvent 40mL acetone and 80mL hexane using ultrasonic agitation for 20 min. Lipids in extracts are degraded by concentrated sulfuric acid and then PBBs are purified with Florisil column. The purified extracts are analyzed by GC/MS-selected ion monitoring mode for the quantitation of PBBs in biota sample. The overall recovery yields of PBBs range between 77 and 111% under these experimental conditions.

Keywords

PBBs;clean-up;biota sample;GC/MS-SIM

Acknowledgement

Supported by : 국립환경연구원

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