Immunohistochemical study of the gastrointestinal endocrine cells in the Mongolian Gerbils, Meriones unguiculatus

몽골리안 저빌(Meriones unguiculatus)의 위장관 내분비 세포에 대한 면역조직화학적 연구

  • Lee, Jae-hyun (Department of Histology, College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Hyeung-sik (Department of Biology, Faculty of Natural Sciences, kyungsan University) ;
  • Ku, Sae-kwang (Pharmacology & Toxicology Lab., Central Research Laboratories, Dong-Wha Pharm. Ind. Co.) ;
  • Park, Ki-dae (Department of Histology, College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Kil-soo (Department of Laboratory Animal Research, Asan Institute for Life Science)
  • 이재현 (경북대학교 수의과대학 조직화교실) ;
  • 이형식 (경산대학교 자연과학부 생물학과) ;
  • 구세광 (동화약품 (주) 중앙연구소 약리독성연구실) ;
  • 박기대 (경북대학교 수의과대학 조직화교실) ;
  • 김길수 (아산생명과학연구소 실험동물연구실)
  • Accepted : 2000.10.20
  • Published : 2000.12.20

Abstract

In order to study the regional distribution and relative frequency of the immunoreactive endocrine cells in the gastrointestinal tract of the Mongolian gerbil, Meriones unguiculatus, the gastrointestinal tract was divided into 9 portions (cardia, fundus, pylorus, duodenum, jejunum, ileum, cecum, colon and rectum) and immunostained by immunohistochemical (PAP) method using 8 types of specific antisera against cholecystokinin (CCK)-8, gastrin, secretin, pancreatic polypeptide(PP), somatostatin, serotonin, glucagon and insulin. CCK-8-, gastrin-, somatostatin- and serotonin-immunoreactive cells were demonstrated in this study. These immunoreactive cells were found in the gastric gland regions of the pylorus or between parietal and chief cells of the fundus with round to spherical shaped, and in the interepithelial regions of the intestinal tract with spherical to spindle shaped except for jejunum where some of immunoreactive cells were also observed in the intestinal glands with round to spherical shaped. CCK-8-immunoreactive cells were restricted to the pylorus and duodenum with numerous and a few frequency, respectively. Gastrin-immunoreactive cells were restricted to the pylorus with numerous frequency. Similar to those of gastrin-immunoreactive cells, somatostatin-immunoreactive cells were restricted to pylorus with moderate frequency. Serotonin-immunoreactive cells were detected throughout whole gastrointestinal tract except for cardia and cecum with moderate or numerous frequency. However, no secretin-, PP-, glucagon- and insulin-immunoreactive cells were observed in this study. From these results, the appearance type, regional distribution and relative frequency of immunoreactive endocrine cells in the gastrointestinal tract of the Mongolian gerbils were somewhat lowered or restricted compared to those of other mammals and these differences were might be caused by feeding habits and species specification.