Quantitation of abamectin by HPTLC and its pharmacokinetics after intramuscular injection in pigs

돼지에서 근육주사한 Abamectin에 대한 HPTLC 분석 및 약물동태학

  • Park, Seung-chun (Department of Veterinary Pharmacology & Toxicology, College of Veterinary Medicine, Chungnam National University) ;
  • Yun, Hyo-in (Department of Veterinary Pharmacology & Toxicology, College of Veterinary Medicine, Chungnam National University)
  • 박승춘 (충남대학교 수의과대학 수의약리독성학교실) ;
  • 윤효인 (충남대학교 수의과대학 수의약리독성학교실)
  • Received : 1999.12.30
  • Published : 2000.03.25

Abstract

We established a new method to analyze abamectin using HPTLC (high performance thin layer chromatography) in order to obtain its pharmacokinetic profiles in pigs. Recovery of abamectin in pig serum after fluorescence derivatization was $80.01{\pm}3.82%$ at 0.1ppm and $83.67{\pm}3.63%$ at 10ppm, respectively. Detection reproducibility in terms of coefficient variation (c.v.) was 3.09% and 2.74% (intra-day), and 3.71% and 51.7%(inter-day), for 0.1 and 10ppm, respectively. Pharmacokinetics of abamectin was studied in five Yorkshire-Landrace mixed bred male pigs ($35.0{\pm}2.7kg$) administered intramuscularly 0.3mg/kg b.w. Pharmacokinetic profiles of abamectin in pigs were described by the 1-compartment open model with first-order absorption and first-order elimination. AUC (area under the curve) was $262.65{\pm}16.44ng{\cdot}day/ml$ and the biological elimination half-life ($t_{1/2},\;k_e$) was $5.28{\pm}0.84$ days, indicating somewhat high bioavailability and long half-life by the intramuscular route. We suggest intramucular injection of abamectin could be also used in place of the recommended route of its subcutaneous administration so far.