Development of competitive enzyme linked immunosorbent assay for detection of Coxiella burnetii antibody in animal

동물에서 Coxiella burnetii 항체를 진단하기 위한 경쟁효소면역법 개발

  • Cho, Dong-hee (National Veterinary Research and Quarantine Service) ;
  • Kim, Yong-ju (National Veterinary Research and Quarantine Service) ;
  • Wee, Sung-hwan (National Veterinary Research and Quarantine Service) ;
  • Cho, Mi-young (National Veterinary Research and Quarantine Service) ;
  • Kweon, Chang-hee (National Veterinary Research and Quarantine Service) ;
  • Kang, Yung-bai (National Veterinary Research and Quarantine Service) ;
  • Park, Yong-ho (College of Veterinary Medicine, Seoul National University) ;
  • Cho, Sang-nae (College of Medicine, Yonsei National University)
  • Received : 2000.01.04
  • Published : 2000.03.25

Abstract

Coxiella burnetii (C burnetii) is the causative agent of Q fever in animal and human. The distribution of the disease has been documented around world. In this study we developed the competitive enzyme linked immunosorbent assay(cELISA) and compared it with indirect immunofluorescent assay(IFA). A monoclonal antibody(Mab) against C burnetii and a peroxidase-conjugated anti-mouse IgM were used as an indicator system competing against antibody in animal serum or as an indicater of the absence of antibody. Sera were considered antibody positive when the percentage inhibition index(PI index) is upper than 30. PI index is calculated as 100-[sample OD/Mab OD)${\times}100$]. Among 162 bovine serum samples, 23 samples were antibody positive both in cELISA and IFA. And 156 samples showed same results. From goat with experimentally induced infection with C burnetii the antibody was detected 20 days early in cELISA compared to IFA. On the basis of present findings, it was demonstrated that cELISA is a reliable diagnostic method for The detection of specific antibodies against C burnetii infection.