The histological and immunohistochemical effects of acua-acupuncture of Bletillae rhizoma extract on the duodenal ulcer induced by HCl-aspirin

백급수침이 HCl-aspirin으로 유발된 십이지장 궤양에 미치는 조직학적 및 면역조직화학적 연구

  • Ku, Seo-kwang (Pharmacology & Toxicology Laboratory, Central Research Laboratories, Dong-Wha Pharm Ind. Co.) ;
  • Lee, Hyeung-sik (Department of Biology, Faculty of Natural Secience, Kyungsan University) ;
  • Kim, Jong-dae (College of Oriental Medicine, Kyungsan University) ;
  • Lee, Jae-hyun (Laboratory of Histology, College of Veterinary Medicine, Kyungpook National University)
  • 구세광 ((주)동화약품 중앙연구소 약리독성연구실) ;
  • 이형식 (경산대학교 자연과학부 생물학전공) ;
  • 김종대 (경산대학교 한의과대학) ;
  • 이재현 (경북대학교 수의과대학 조직학교실)
  • Received : 1999.12.28
  • Published : 2000.03.25


To identify, the effects of acua-acupuncture of Bletillae rhizoma extract on the duodenal ulcer induced by HCl-aspirin in rats, the changes of histological profiles, number of mucous-producing goblet cells (PAS-positive cells), and morphology and/or number of chole-cystokinin (CCK)-8 and serotonin-immunoreactive (IR) cells in the gastrointestinal tract were observed after acua-acupuncture of Bletillae rhizoma extract on Ganshu locus ($BL_{14}$). Samples were collected at 1, 3 and 5 days after treatment. Histologically, very severe injury, atrophy of villus, necrosis of epithelial cell and hemorrhage, to the duodenal mucosa including epithelium were observed in HCl-aspirin administrated control groups, and these injuries were increased with time intervals. But in acua-acupuncture groups and nontreated normal groups, no gross lesion of ulcer was demonstrated and histologically minor injury to the apex of villas epithelium was observed. Compared to the PAS-positive cells of the control groups, those of the acua-acupuncture groups were increased. Severe degranulation of CCK-8- and serotonin-IR cells were observed in the control groups but these phenomena seldom occur in the acua-acupuncture groups. Serotonin-IR cells were significantly decreased in control groups but increased in the acua-acupuncture groups compared to control groups. However, these degranulation of IR cells of the aqua-acupuncture groups were less severe than those of normal groups, and number of serotonin-IR cells were lower than those of normal groups but these phenomena were decreased with time intervals and in 5 days after treatment, they were observed like those of normal groups. These result indicated that acua-acupuncture of Bletillae rhizoma extract would be accelerated the healing of the duodenal ulcer but the functional mechanisms were unknown.