Immunohistochemical study of CPP32 (Caspase-3) in the spinal cords of rats with experimental autoimmune encephalomyelitis

자기면역성 뇌척수염 조직에서 CPP32의 면역조직화학적 관찰

  • Shin, Tae-kyun (Department of Veterinary Medicine, Institute for Life Science, Brain Korea 21, Cheju National University) ;
  • Moon, Chang-jong (Department of Veterinary Medicine, Institute for Life Science, Brain Korea 21, Cheju National University) ;
  • Ahn, Mee-jung (Department of Veterinary Medicine, Institute for Life Science, Brain Korea 21, Cheju National University) ;
  • Wie, Myung-bok (Department of Veterinary Medicine, Institute for Life Science, Brain Korea 21, Cheju National University)
  • 신태균 (제주대학교 농과대학 수의학과, 생명과학연구소, Brain Korea 21) ;
  • 문창종 (제주대학교 농과대학 수의학과, 생명과학연구소, Brain Korea 21) ;
  • 안미정 (제주대학교 농과대학 수의학과, 생명과학연구소, Brain Korea 21) ;
  • 위명복 (제주대학교 농과대학 수의학과, 생명과학연구소, Brain Korea 21)
  • Received : 2000.07.13
  • Published : 2000.09.25

Abstract

The aim of this study was to evaluate the involvement of CPP32 (caspase-3), one of the death-related enzymes, in the course of experimental autoimmune encephalomyelitis (EAE). EAE was induced in Lewis rats immunized with an emulsion of rat spinal cord homogenate with complete Freunds adjuvant supplemented with Mycobacterium tuberculosis (H37Ra, 5mg/ml). The expression of CPP32 in the spinal cords of rats with EAE was studied. In normal rat spinal cords, CPP32 is constitutively, but weakly, expressed in neurons and some neuroglial cells. In the EAE spinal cords, many inflammatory cells were positive for CPP 32, and the majority of CPP32(+) cells were identified as ED1(+) macrophages. During this stage of EAE, the number of CPP32(+) cells in brain cells, including neurons and astrocytes, increased, and these cells also had increased CPP32 immunoreactivity. CPP32 immunor eactivity was not always matched with apoptosis of inflammatory cells in EAE lesions. We speculate that CPP32, which is constitutlvely expressed in brain cells, increases in response to neuroimmunological stimulation in both brain neuronal cells and inflammatory cells. The functional role of CPP32 in neuroimmunological disorders is discussed.