Study for comparison with male & female rat locus coeruleus using confocal laser scanning microscopy

공초점현미경을 이용한 암수 흰쥐 청색반점의 비교연구

  • Park, Il-kwon (Department of History, College of Veterinary Medicine, Chungnam National University) ;
  • Song, Chi-won (Department of History, College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Kyung-youl (Department of History, College of Veterinary Medicine, Chungnam National University) ;
  • Kwon, Hyo-jung (Department of History, College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Moo-kang (Department of History, College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Kang-iee (College of Oriental Medicine, Taejon University) ;
  • Jeong, Young-gil (Department of Anatomy, College of Medicine Gonyang University) ;
  • Lee, Nam-seob (Department of Anatomy, College of Medicine Gonyang University) ;
  • Ha, Kwon-soo (Korea Basic Science Institute)
  • 박일권 (충남대학교 수의과대학 조직학교실) ;
  • 송치원 (충남대학교 수의과대학 조직학교실) ;
  • 이경열 (충남대학교 수의과대학 조직학교실) ;
  • 권효정 (충남대학교 수의과대학 조직학교실) ;
  • 김무강 (충남대학교 수의과대학 조직학교실) ;
  • 이강이 (대전대학교 한의과대학) ;
  • 정영길 (건양대학교 의과대학 해부학교실) ;
  • 이남섭 (건양대학교 의과대학 해부학교실) ;
  • 하권수 (기초과학자원연구소)
  • Received : 2000.08.03
  • Published : 2000.09.25

Abstract

The locus coeruleus(LG) is known to be observed a sexual dimorphism in rat CNS. LC is the largest collections of norepinephrine(NE)-containing neurons in the mammalian brain. Especially in rat, all LC neurons contained NE unlike other mammalians, so that specific reactions were found in the tyrosine-hydroxylase(TH) immunoreactive neurons. Sexual dimorphism of rat LC has affected by genes before sex hormone appeared, thereafter affected by sex hormones. In these day, many scientists founded morphological differences between male and female LC morphology, but differences of entire structure was not founded. Thus we investigated sex differences of the LC neuron's morphology in rat by three-dimensional(3-D) reconstruction using Confocal laser scanning microscopy(CLSM). We reported that neuron's shape was relatively-large multipolar neurons and neuron's processes in dorsal LC proceeded to ventral direction in the male and female rat. Male had a longer anterior-posterior length than female had in dorsal LC. In addition to middle-LC, male rat's LC had a more thicker posterior region but had not viewed in a previous study. In reverse, female rat's LC had a thicker anterior region like a previous study. This results using 3-D reconstruction by CLSM showed that the male's LC was more wide-ranging than female's relatively.