Tracing study for the rabbit eye ball control nerve utilizing the PRV-Ba isolated in the Korea

한국분리산 PRV-Ba를 이용한 가토 안구지배신경의 추적 연구

  • Park, Il-kwon (College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Moo-kang (College of Veterinary Medicine, Chungnam National University) ;
  • Shin, Kwang-soon (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Kyung-youl (College of Veterinary Medicine, Chungnam National University) ;
  • Song, Chi-won (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Kang-iee (College of Oriental Medicine, Taejon University) ;
  • Hyun, Byung-hwa (Korea Research Institute of Bioscience & Biotechnology) ;
  • Chang, Kyu-tae (Korea Research Institute of Bioscience & Biotechnology) ;
  • Jeong, Young-gil (College of Medicine, Gonyang University)
  • Received : 2000.08.03
  • Published : 2000.09.25

Abstract

Until now peudorabies virus(PRV) has been used a neurotracer, because of it's properties of retrograde & anterograde transport. But it's anterograde transfort is not perfect, so we tested the applicability of the Bartha strain of PRV(PRV-Ba) isolated from South Korea as a neurotracer in the visual system. We performed immunohistochemical study of the rabbit brain after intravitreal injection of the PRV-Ba. After given survival time(24, 48, 72, 96, 120, 144hrs), the brain was removed and processed immunohistochemical stain for PRV-Ba. The strong PRV immunoreactivity(PRV-ir) were almost oberserved contralaterally in oculomotor neurons, fro example Edinger-Westphal nucleus, trigerminal nucleus of pons and peritrigerminal zone but locus of innervating sensitive neurons. The latter were weak positive and selective. PRV-Ba immunoreactive neurons were stained strongly in nucleus compared to cytoplasm. This study suggests that PRV-Ba isolated from South Korea is also a useful neurotracer in the motor innervated system like other PRV-strain.