Effects of hyperbaric oxygen and α-tocopherol on full-thickness skin grafts in rats

쥐(rats)의 full-thickness skin grafts에 대한 hyperbaric oxygen과 α-tocopherol의 효과

  • Kim, Jong-shu (College of Veterinary Medicine, Gyungsang National University(Institute of Animal Medicine)) ;
  • Kim, Chung-hul (Department of Microbiology, College of Medicine, In-Je University) ;
  • Kim, Gon-sup (College of Veterinary Medicine, Gyungsang National University(Institute of Animal Medicine)) ;
  • Hah, Dae-sik (Gyeongnam Provincial Government Institute of Health and Environment) ;
  • Park, Sun-gun (Department of Plastic Surgery, College of Medicine, Gyungsang National University) ;
  • Kim, Yang-mi (Department of Physiology and Biophysics, Chicago Medical School, Finch University of Health Sciences)
  • 김종수 (경상대학교 수의과대학) ;
  • 김충희 (인제대학교 의과대학 미생물학교실) ;
  • 김곤섭 (경상대학교 수의과대학) ;
  • 하대식 (경상남도 보건환경연구원) ;
  • 박성근 (경상대학교 의과대학 성형외과) ;
  • 김양미 (미국 Chicago 의과대학 생물학교실)
  • Received : 2000.08.22
  • Published : 2000.09.25


To document that effects of hyperbaric oxygen(HBO) and ${\alpha}-tocopherol$ on full-thickness skin grafts in rat, we performed full-thickness skin grafts bilaterally on each rats. The HBO-treated rats were received HBO twice daily for 90 minutes at 2 ATA. Surgical control rats were not treated with HBO. ${\alpha}-tocopherol$ treated rats were received the agent via oral gastric tube daily for 3 days preoperative and a fourth dose 1 to 2 hours postoperative. HBO plus ${\alpha}-tocopherol$ treated rats were received HBO and ${\alpha}-tocopherol$ as mentioned above. Biopsy specimens were taken from each rat at the time of grafting and on days 2, 4, 7, 10, 14, 21, and 28, then were processed for tissue-concentration of total glutathione(GSHt), oxidized/reduced glutathione level, and thiobarbituric acid-reactive substance(TBARS) levels. The percentage of viable graft on day 10 ranged from 67 to 93%, and was not significantly different among the each other groups. The percentage of viable graft were, however, higher in HBO plus ${\alpha}-tocopherol$ treated rats(78.6%) than in HBO alone treated rats(59.1%), ${\alpha}-tocopherol$ alone treated rats(66.7%) and surgical control rats(58.2%). TBARS concentration had a significant increase from preoperative concentration at day 2, and peak concentration at day 4(p<0.01). Concentration then decreased to preoperative concentration at day 28. GSHt concentration of free skin graft had a similar patteren of change in four groups and decreased significantly from preoperative concentration at day 2, returning to preoperative concentration by day 7(surgical control, HBO-treated, and ${\alpha}-tocopherol-treated$, alone) and 28(HBO plus ${\alpha}-tocopherol-treated$). Percentage of the concentration of reduced glutathione decreased in surgical control, HBO-treated and, ${\alpha}-tocopherol-treated$(p<0.05), and HBO plus ${\alpha}-tocopherol-treared$(p<0.01) on day 7 after surgery, whereas the concentration of oxidized increased significantly in HBO-treated(p<0.05), ${\alpha}-tocopherol-treated$(p<0.05), and HBO plus ${\alpha}-tocopherol-treated$(p<0.01).