Development of an ELISA kit for the detection of residual sulfadimethoxine in edible animal products

축산물 잔류 sulfadimethoxine 검출용 ELISA kit 개발

  • Kim, Woo-taek (Cheju Institute for Livestock Promotion) ;
  • Kim, Seong-hee (Department of Veterinary Medicine, Cheju National University) ;
  • Yoon, Byoung-su (Department of Biology, College of Natural Science Kyonggi University) ;
  • Lim, Yoon-kyu (Department of Veterinary Medicine, Cheju National University)
  • 김우택 (제주도 축산진흥원) ;
  • 김성희 (제주대학교 농과대학 수의학과) ;
  • 윤병수 (경기대학교 이과대학 생물학과) ;
  • 임윤규 (제주대학교 농과대학 수의학과)
  • Received : 2000.08.03
  • Published : 2000.09.25

Abstract

An enzyme linked immunosorbent assay (ELISA) was developed to screen residues of sulfadimethoxine (SDM) in edible animal products. An indirect competitive ELISA was allowed to compete with rabbit anti-SDM for binding to a limited amount of SDM-gelatin conjugate and SDM in serum samples. Sera was diluted 20 times with phosphate buffered saline (PBS) and boiled for 5 minutes to destruct immunoglobulins of serum. Detection limit of this competitive ELISA for SDM was 0.1 ppb or less. Among eight sulfonamide analogues tested for specifity, only sulfamonomethoxine showed significant cross-reaction in the assay. The EC-50 value for sulfamonomethoxine was 3.5 ppm. Recovery of SDM in spiked serum samples between 100 ppb and 500 ppb ranged from 110.7% to 128.9%.