Purification and Characterization of Lysyl Oxidase from Fetal Bovine Aorta in the presence of protease inhibitors -Evidence against polymorphism-

소(牛) 태아(胎兒) 대동맥(大動脈)으로부터 단백 분해효소 억제제 존제 하에 Lysyl Oxidase의 순수분리(純棒分離) -다양성(多形性)에 대(對)한 반론(反論)-

  • Han, Song (Department of Biochemistry, College of Dentistry, Kangnung National University)
  • 한송 (강릉대학교 치과대학 구강생화학교실)
  • Published : 2000.03.30

Abstract

Lysyl Oxidase from fetal bovine aorta was purified to homogenity using extraction, Sephacryl S200HR chromatography, Hydropore AX ion-exchange high performance liquid column chromatography, Cibacron blue affinity chromatography, and Sephacryl S-300 HR chromatography in the presence of protease inhibitor. The purified enzyme was active toward lathyritic collagen as well as elastin and was sensitive to aminonitriles such as BAPN. Upon Sephacryl S-300 HR chromatography, the enzyme was eluted as a peak with a $K_{av}$ value of 0.45 (65% of $V_t$ ) and it eluted from high performance liquid ion-exchange column (Hydropore |AX) at single position (ionic strength, I = 0.1~0.15). Once purified, it showed one band upon SDS-PAGE. It migrated to a band the mobility of which corresponded to a Mr of 33,500 upon reduction while it migrated to a 24,500 Mr position under the non-reducing condition. In constrast to other reports, it is concluded that fetal bovine aorta contains only one type of lysy oxidase.

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