The Effects of Cyclooxygenase-2(COX-2) Inhibitor on COX-2 and Prostaglandin E2 Expression in Ovalbumin Induced Early Phase Bronchoconstriction of Rats

Ovalbumin으로 유발된 백서의 즉시형 기관지 수축 반응에서 Cyclooxygenase-2(COX-2) 발현 양상 및 혈중 프로스타글란딘 E2 농도와 COX-2 억제제의 효과

  • Lee, Sung-Yong (Department of Internal Medicine, Korea University College of Medicine) ;
  • Lee, Sin-Hyung (Department of Internal Medicine, Korea University College of Medicine) ;
  • Jung, Ki-Hwan (Department of Internal Medicine, Korea University College of Medicine) ;
  • Kim, Byung-Gyu (Department of Internal Medicine, Korea University College of Medicine) ;
  • Jung, Hae-Chul (Department of Internal Medicine, Korea University College of Medicine) ;
  • Kim, Kyung-Kyu (Department of Internal Medicine, Korea University College of Medicine) ;
  • Kwon, Young-Hwan (Department of Internal Medicine, Korea University College of Medicine) ;
  • Kim, Ja-Hyeong (Department of Internal Medicine, Korea University College of Medicine) ;
  • Lee, Ju-Han (Department of Pathology, Korea University College of Medicine) ;
  • Lee, Sang-Youb (Department of Internal Medicine, Korea University College of Medicine) ;
  • Cho, Jae-Yoen (Department of Internal Medicine, Korea University College of Medicine) ;
  • Shim, Jae-Joeng (Department of Internal Medicine, Korea University College of Medicine) ;
  • In, Kwang-Ho (Department of Internal Medicine, Korea University College of Medicine) ;
  • Yoo, Se-Hwa (Department of Internal Medicine, Korea University College of Medicine) ;
  • Kang, Kyung-Ho (Department of Internal Medicine, Korea University College of Medicine)
  • 이승룡 (고려대학교 의과대학 내과학교실) ;
  • 이신형 (고려대학교 의과대학 내과학교실) ;
  • 정기환 (고려대학교 의과대학 내과학교실) ;
  • 김병규 (고려대학교 의과대학 내과학교실) ;
  • 정혜철 (고려대학교 의과대학 내과학교실) ;
  • 김경규 (고려대학교 의과대학 내과학교실) ;
  • 권영환 (고려대학교 의과대학 내과학교실) ;
  • 김제형 (고려대학교 의과대학 내과학교실) ;
  • 이주한 (고려대학교 의과대학 병리학교실) ;
  • 이상엽 (고려대학교 의과대학 내과학교실) ;
  • 조재연 (고려대학교 의과대학 내과학교실) ;
  • 심재정 (고려대학교 의과대학 내과학교실) ;
  • 인광호 (고려대학교 의과대학 내과학교실) ;
  • 유세화 (고려대학교 의과대학 내과학교실) ;
  • 강경호 (고려대학교 의과대학 내과학교실)
  • Published : 2000.02.29

Abstract

Background: Bronchial asthma is characterized by airway hyperresponsiveness(BHR) and inflammation. The cyclooxygenase(COX) is believed to be one of the important enzymes in these inflammatory reactions. Recently, the COX was divided into two isoforms, COX1 and COX2. COX2 is induced by lipopolysaccharide and some cytokines at the inflammation site. Prostaglandin E2(PGE2), produced from COX2, may affect airway inflammation. The purpose of this study is to evaluate the effect of COX2 inhibitor on COX2 expression, plasma PGE2, airway resistance and histologic finding in an animal asthma model. Methods : Sprague-Dawley rats were divided into 3 groups. The normal control group did not receive any treatment, but the asthma control group was sensitized by ovalbumin but not treated with the COX2 inhibitor(nimesulide, Mesulid$^{(R)}$). The treatment group was sensitized and treated with nimesulide. Specific airway resistance(sRaw) before and after nimesulide ingestion was investigated. The PGE2 level in the plasma was examined and COX2 immunogold-silver stain on lung tissue was performed. Results: sRaw and eosionophilic infiltration on airway, which increased in the asthma control group, was compared to normal control(p=0.014). However, there was no difference in eosinophilic infiltration between asthma control and treatment groups(p=0.408) and no difference in COX2 expression on bronchiolar epithelium among the three groups. Plasma PGE2 levels were not statically different among the three groups. Conclusion: The role of COX2 in the allergen-induced BHR was not significant The effect of nimesulide was not observed on BHR, COX2 expression, and plasma PGE2 level. Therefore, COX2 may not be a major substance of allergic asthma.