Perfluorocarbon Does Not Inhibit Chemokine Expression in Airway Epithelial Cells

Perfluorocarbon이 기도 상피세포 Chemokine 발현에 미치는 영향에 관한 연구

  • Suh, Gee-Young (Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Samsung Biomedical Research Institute) ;
  • Kang, Kyeong-Woo (Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Samsung Biomedical Research Institute) ;
  • Park, Sang-Joon (Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Samsung Biomedical Research Institute) ;
  • Chung, Man-Pyo (Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Samsung Biomedical Research Institute) ;
  • Kim, Ho-Joong (Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Samsung Biomedical Research Institute) ;
  • Choi, Dong-Chull (Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Samsung Biomedical Research Institute) ;
  • Rhee, Chong-H (Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Samsung Biomedical Research Institute) ;
  • Kwon, O-Jung (Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Samsung Biomedical Research Institute)
  • 서지영 (성균관대학교 의과대학 내과학교실, 삼성서울병원 호흡기내과, 삼성생명과학연구소) ;
  • 강경우 (성균관대학교 의과대학 내과학교실, 삼성서울병원 호흡기내과, 삼성생명과학연구소) ;
  • 박상준 (성균관대학교 의과대학 내과학교실, 삼성서울병원 호흡기내과, 삼성생명과학연구소) ;
  • 정만표 (성균관대학교 의과대학 내과학교실, 삼성서울병원 호흡기내과, 삼성생명과학연구소) ;
  • 김호중 (성균관대학교 의과대학 내과학교실, 삼성서울병원 호흡기내과, 삼성생명과학연구소) ;
  • 최동철 (성균관대학교 의과대학 내과학교실, 삼성서울병원 호흡기내과, 삼성생명과학연구소) ;
  • 이종헌 (성균관대학교 의과대학 내과학교실, 삼성서울병원 호흡기내과, 삼성생명과학연구소) ;
  • 권오정 (성균관대학교 의과대학 내과학교실, 삼성서울병원 호흡기내과, 삼성생명과학연구소)
  • Published : 2000.02.29

Abstract

Background: Liquid ventilation is associated with decreased inflammatory response in an injured lung. This study was performed to investigate if whether perfluorocarbon(PFC) can decrease chemokine expression in airway epithelial cells. Methods: A549 cells were used for airway epithelial cells and perfluorodecalin for PFC. To expose cells to PFC, lower chamber of Transwell$^{(R)}$plate was used. This study was performed in two parts. In the first part, we examined whether PFC could decrease chemokine expression in airway epithelial cells through inhibition of other inflammatory cells. Peripheral blood mononuclear cells(PBMC's) were isolated and stimulated with lipopolysaccharide(LPS, 10 ${\mu}g/mL$) for 24 hours with or without exposure to PFC. Then A549 cells were stimulated with conditioned media(CM) containing the culture supernatants of PBMC. After 24 hours, the expressions of interleukin-8(IL-8) and RANTES were measured. In the second part of the study, we studied whether PFC could directly suppress chemokine expression in airway epithelial cells. A549 cells were stimulated for 24 hours with interleukin-l$\beta$ and/or tumor necrosis factor-$\alpha$ with or without exposure to PFC, and then the chemokine expression was measured. Northern analysis was used to measure the mRNA expression, and ELISA was used for immunoreactive protein measurements in culture supernatant. Results: 1. IL-8 and RANTES mRNA expression and immunoreactive protein production were increased significantly by CM from LPS-stimulated PBMC in A459 cells compared to with CM from unstimulated PBCM (p<0.05), but exposure of PFC had no significant effect on either mRNA expression or immunoreactive protein expression. 2. IL-8 and RANTES mRNA expression and immunoreactive protein production were increased significantly by IL-1$\beta$ and TNF-$\alpha$ in A549 cells(p<0.05), but exposure of PFC had no significant effect on neither either mRNA expression nor immunoreactive protein production. Conclusion : Decreased chemokine expression of airway epithelial cells may not be involved in decreased inflammatory response observed in liquid ventilation. Further studies on possible mechanisms of decreased inflammatory response are warranted.

Acknowledgement

Supported by : 보건복지부