Induction of NAD(P)H:quinone reductase and glutathione S-transferase by Xanthii Fructus and Prunellae Spica Extracts

창이자 및 꿀풀하고초에 의한 NAD(P)H:quinone reductase와 glutathione S-transferase의 유도

  • Shon, Yun-Hee (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University) ;
  • Lee, Ki-Taek (Department of AM-Pointology, College of Oriental Medicine, Dongguk University) ;
  • Park, Sin-Hwa (Department of AM-Pointology, College of Oriental Medicine, Dongguk University) ;
  • Cho, Kyoung-Hee (Department of AM-Pointology, College of Oriental Medicine, Dongguk University) ;
  • Lim, Jong-Kook (Department of AM-Pointology, College of Oriental Medicine, Dongguk University) ;
  • Nam, Kyung-Soo (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University)
  • 손윤희 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구센터) ;
  • 이기택 (동국대학교 한의과대학 경혈학교실) ;
  • 박신화 (동국대학교 한의과대학 경혈학교실) ;
  • 조경희 (동국대학교 한의과대학 경혈학교실) ;
  • 임종국 (동국대학교 한의과대학 경혈학교실) ;
  • 남경수 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구센터)
  • Published : 2001.12.30

Abstract

Ethanol extracts from Xanthii Fructus (XFE) and Prunellae Spica (PSE) were investigated for the effects on the induction of cancer chemoprevention-associated enzymes. The following effects were measured: (a) induction of quinone reductase (QR) (b) induction of glutathione S-transferase (GST) (c) reduced glutathione (GSH) level. XFE and PSE were potent inducers of quinone reductase activity in Hepa1c1c7 murine hepatoma cells. Glutathione levels were increased with XFE and PSE. In addition, glutathione S-transferase activity was increased with XFE. However, GST activity was not increased with PSE. These results suggest that XFE and PSE have chemopreventive potentials by inducing quinone reductase and increasing GSH levels.