Effect of Dietary Mulberry leaf on the Composition of Intestinal Microflora in SD Rats

식이 뽕잎이 흰쥐의 장내균총 조성에 미치는 영향

  • Lee, Heui-Sam (Department of Sericulture and Entomology, NIAST, RDA) ;
  • Jeon, Ho-Jung (College of Agriculture and Life Science, Seoul National University) ;
  • Lee, Sang-Duk (College of Agriculture and Life Science, Seoul National University) ;
  • Moon, Jae-Yu (College of Agriculture and Life Science, Seoul National University) ;
  • Kim, Ae-Jung (Department of Food & Nutrition, Hyejeon College) ;
  • Ryu, Kang-Sun (Department of Sericulture and Entomology, NIAST, RDA)
  • 이희삼 (농업과학기술원 잠사곤충부) ;
  • 전호정 (서울대학교 농업생명과학대학) ;
  • 이상덕 (서울대학교 농업생명과학대학) ;
  • 문재유 (서울대학교 농업생명과학대학) ;
  • 김애정 (혜전대학) ;
  • 류강선 (농업과학기술원 잠사곤충부)
  • Published : 2001.04.30

Abstract

This study was performed to investigate the influence of dietary mulberry leaf on the intestinal microflora in rats. Rats were fed each experimental diets containing 1%, 10% of mulberry leaf powder for 4 weeks. Total viable counts and the numbers of Bifidobacterium, Lactobacillus, Clostridium, E. coli and Staphylococcus were determined by nonselective media and various selective media. A decrease in the intestinal population of Clostridium was shown in dietary mulberry leaf group. The E. coli and Staphylococcus populations decreased in dietary mulberry leaf group compared with control group. Methanol extract and fractions of mulberry leaf were subjected to an in vitro screening test for their growth-inhibitory activity. Methanol extract and Water fraction of Mulberry leaves showed weak growth-inhibition of Clostridium perfringens. These results indicate that the composition of gastrointestinal microflora was improved by treatment of mulberry leaves in SD rats and was very effective for growth inhibition of the intestinal harmful bacteria in intestine. Therefore, the mulberry leaves as a newly bio-material can be a useful material for physiologically functional food.

Keywords

mulberry leaf;intestinal microflora