The Difference of Ginsenoside Compositions According to the Conditions of Extraction and Fractionation of Crude Ginseng Saponins

추출 및 분획조건에 따른 인삼 조사포닌 중 ginsenoside 조성 차이

  • Shin, Ji-Young (Department of Food Microbial Technology Graduate School, Seoul Women's University) ;
  • Choi, Eon-Ho (Department of Food Microbial Technology Graduate School, Seoul Women's University) ;
  • Wee, Jae-Joon (Korea Ginseng and Tobacco Research Institute)
  • 신지영 (서울여자대학교 식품미생물 공학과) ;
  • 최언호 (서울여자대학교 식품미생물 공학과) ;
  • 위재준 (한국인삼연초연구원)
  • Published : 2001.06.30

Abstract

This study was carried out to investigate the difference of ginsenoside compositions in crude ginseng saponins prepared by five different methods including three new methods. Two known methods are hot methanol(MeOH) extraction/n-butanol(n-BuOH) fractionation and hot MeOH extraction/Diaion HP-20 adsorption/MeOH elution. Three new methods are hot MeOH extraction/cation AG 50W $absorption/H_2O$ elution/n-BuOH extraction, cool MeOH extraction/Diaion HP-20 adsorption/MeOH elution and direct extraction with ethyl acetate(EtOAc)/n-BuOH. Analysis of ginsenoside composition in the crude saponins by conventional HPLC/RI(Refractive Index) did not show great difference between methods except EtOAc/n-BuOH method. However, HPLC/ELSD (evaporative light scattering detector) employing gradient mobile phase afforded fine resolution of ginsenoside Rf, $Rg_1$ and $Rh_1$, and great difference of ginsenoside compositions between methods. LC/MS revealed that large amount of prosapogenins were produced during the pass through the cation exchange (AG 50W) column being strongly acidic. Six major ginsenosides such as $Rb_1,w;Rb_2,$ Rc, Rd, Re and $Rg_1$, 5 prosapogenins and one chikusetsusaponin were identified by LC/MS. A newly established HPLC method employing ODS column and gradient mobile phase of $KH_2PO_4/CH_3CN$ revealed that malonyl ginsenosides were detected only in the crude saponin obtained from cool MeOH extraction.

Keywords

crude ginseng saponin;ginsenoside;prosapogenin;malonyl ginsenosides;LC/MS