The Effect of Antioxidant Vitamins on Aflatoxin $B_{1}-DNA$ Adduct the Formation in Aflatoxin $B_1$ Administered Mice Liver

항산화비타민이 Aflatoxin $B_1$ 투여 마우스의 간세포에서 Aflatoxin $B_{1}-DNA$ Adduct 형성에 미치는 영향

  • Park, Seon-Ja (Division of Applied Life Science, Gyeongsang National University) ;
  • Kang, Sung-Jo (Division of Applied Life Science, Gyeongsang National University) ;
  • Park, Jung-Hyun (Division of Applied Life Science, Gyeongsang National University) ;
  • Oh, Sang-Suk (Department of Food and Nutrition, Ewha Womans University) ;
  • Chung, Duck-Hwa (Division of Applied Life Science, Gyeongsang National University)
  • 박선자 (경상대학교 응용생명과학부) ;
  • 강성조 (경상대학교 응용생명과학부) ;
  • 박정현 (경상대학교 응용생명과학부) ;
  • 오상석 (이화여자대학교 식품영양학과) ;
  • 정덕화 (경상대학교 응용생명과학부)
  • Published : 2001.12.01

Abstract

The objective of this study was to examine the effects of antioxidant vitamins on the formation of $AFB_{1}-DNA$ adduct and $AFB_{1}-inducing$ cellular oxidative damage. Intraperitoneal(i.p.)injections of 10 mg/kg vitamin C(VC) and 63.8 mg/kg vitamin E(VE) were repeatedly administrated 4 times with 2 days interval to 6 week old male ICR mice. After one hour of vitamin treatments, 0.4 mg/kg $AFB_1$ was injected in $AFB_1$ plus vitamin treated groups by same way. On the other hands, $AFB_1$ treated group was only injected with $AFB_1$ by the same method described above without vitamins. According to quantitative analysis of the $AFB_1$ in mice serum by indirect competitive ELISA, 12.28 and 18.78 ng/mL were detected in $AFB_1-treated$ groups, but 7.60 and 4.85 ng/mL in $AFB_1$ plus VC and VE treated groups, respectively. 23.78, 25.48 ng/mL of $AFB_1-DNA$ adduct were detected in mice liver of $AFB_1$treated groups, while 5.26, 7.81 ng/mL in $AFB_1$ plus VC and VE treated groups, respectively. Consequently, the differences in the concentrations of $AFB_1$ related materials between vitamin treated and non-treated groups were significant. Immunohistochemistry revealed brownish infiltration of $AFB_1$ around central vein and sinusoid in $AFB_1-treated$ group. This manifestation was distinctly reduced in $AFB_1$ plus VC and VE treated groups.