The Study on Acute°§Subacute Toxicity of Juglandis Semen Herbal-acupuncture(JsD)

호도약침(胡桃藥鍼)의 급성(急性) 아급성(亞急性) 독성(毒性)에 관(關)한 실험적(實驗的) 연구(硏究)

  • Published : 2001.12.30


Objective : This study was purposed to investigate the acute. subacute toxicity of Herbal acupuncture with Juglandis Semen(JsD) in mice and rats. Methods & Results: Balb/c mice were injected intraperitoneally with JsD for $LD_{50}$ and acute toxicity test Sprague-Dawley rats were injected intraperitoneally with JsD for subacute toxicity test. Results: The results obtained were summarized as follows; 1. LD50 was uncountable as could not find the expired of treat group. 2. The clinical signs and body weight changes of mice treated with 0.2cc, 0.4cc JsD were not affected during the acute toxicity test. 3. In acute toxicity test of serum biochemical values of mice, total protein was increased in treat-l group, compared with normal group, and total cholesterol was increased in treat-2 group, compared with normal group.(P<0.05) 4. In subacute toxicity test, main toxic syndrome was not found. 5. The body weight was decreased in treat-2 group, compared with normal group and relative liver weight was decreased in treat-1, 2 group, compared with normal group in subacute toxicity test.(P<0.05) 6. In subacute toxicity test, WBC, MCH, MCHC were decreased in treat-2 group and RBC was increased in treat-2 group, compared with normal group in complete blood count test.(P<0.05) 7. In subacute toxicity test, treat groups were not changed serum biochemical values of rats, compared with normal group.(P<0.05) Conclusions: According to the results, Herbal-acupuncture with Juglandis Semen caused no toxicity.


Juglandis Semen;Herbal-acupuncture;$LD_{50}$;Acute toxicity;Subacute toxicity


  1. 약침요법 시술 지침서 대한약침학회
  2. The 1'st Journal of International Congress of KIHA v.4 no.1 KGMP를 대비한 국내약침제재의 조제현황과 미생물 검사보고 강대인;권기록
  3. 本草學 全國韓醫科大學 本草學敎授(編)
  4. 中草藥學 上海中醫學院(編)
  5. 慶熙韓醫大論文集 v.10 荏油 및 胡桃油水鍼이 鎭痛效果에 미치는 影響 吉寸永星;姜成吉
  6. 大韓韓醫學會誌 v.17 no.1 胡桃藥鍼液이 抗酸化 效果에 對한 硏究 金永海;金甲成
  7. 大韓鍼灸學會誌 v.16 no.3 胡桃藥鍼液이 水銀에 의한 肝組織損傷에 미치는 影響 李京泰;宋春浩
  8. 식품의약품안전본부 고시(1998. 12. 3제정)의 '의약품 독성시험기준'제 98-116호
  9. A foundation for analysis in the health sciences Daniel, W.W.
  10. 本草求眞 黃官繡
  11. 本草綱目精要 湖北省中醫藥硏究院醫史文獻硏究室
  12. 完譯 中藥大辭典 金昌玟(外)
  13. 鍼灸學(上.下) 崔容泰
  14. 大韓鍼灸學會誌 鹿茸 및 靈芷水鍼의 急性毒性에 關한 實驗的 硏究 曺珣鄕(外)
  15. J. Life Resources & Industry v.4 마우스에서 애엽 약침액의 급성독성 실험 조경희(외)
  16. 胡桃藥鍼이 Glycerol에 急性腎不全 誘發時 家토의 尿濃縮能의 障碍에 대한 影響 v.18 no.3 李炳薰

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